English
Hujayra madaniyati flasksbozorda keng qo'llaniladigan hujayra madaniyati sarf materiallarining bir turi bo'lib, asosan yopishqoq hujayra madaniyati uchun mos keladi. Ular orasida ushbu sarf moddasi sichqonchaning fibroblastlarini o'tishda ham qo'llaniladi.
Sichqoncha fibroblastlari ilmiy tadqiqot yoki sanoat ilovalari kabi tibbiy bo'lmagan maqsadlarda ishlatiladigan maxsus hujayralardir. Fibroblastlar kuchli funktsiyalarga va zaif bazofil sitoplazmaga ega. Ular aniq oqsil sintezi va sekretsiya faolligiga ega. Muayyan sharoitlarda ular tolali hujayralar bilan o'zaro transformatsiyaga erishishlari mumkin. Fibroblastlarni qabul qilgandan so'ng, ular taxminan 4 soat davomida inkubatorda turg'un bo'lishi kerak, so'ngra muhitni o'zgartirib, hujayra zichligiga qarab o'stirilishi yoki o'tishi kerak. O'tkazish operatsiyasi quyidagi bosqichlardan iborat:
1. Asl hujayra madaniyati kolbasida muhitni aspiratsiya qiling, hujayralarni ikki marta PBS buferi bilan yuving va tripsin hazm qilish uchun 1 ~ 2 ml 0,25% EDTA qo'shing (hazm qilish vaqtiga e'tibor bering, odatda 1 ~ 2 minut ichida nazorat qilinadi).
2. Mikroskop ostida ovqat hazm qilishni kuzating. Hujayra qirrasi qisqarganda va devorga yopishganda (hujayralar suzmaguncha ovqat hazm qilish tavsiya etilmaydi), tripsinni olib tashlang, 6 ~ 8 ml to'liq vosita qo'shing, hujayra qatlamini muloyimlik bilan puflang va hujayra qatlamini puflab, puflashga harakat qiling. .
3. Hujayra suspenziyasining bir qismini oling va uni yangi hujayra kulturasiga o'tkazing, tegishli to'liq muhitni qo'shing va inkubatorda kultivatsiya qiling.
4. Muhitning pH qiymatining o'zgarishiga e'tibor bering, muhitni muntazam ravishda o'zgartiring va hujayra zichligi 70-80% ga etganida subkulturani takrorlang yoki muzlatib qo'ying.
Hujayra o'tishida hujayra madaniyati kolbalari ning asosiy roli hujayralarni saqlaydi va hujayralar uchun yopishqoq o'sish muhitini ta'minlaydi. Shuni ta'kidlash kerakki, hujayralarni qabul qilgandan so'ng hujayra devori singan yoki oqayotgan bo'lsa, bu hujayralar ifloslanganligini anglatadi va etkazib beruvchiga o'z vaqtida murojaat qilish kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.