Principle of cell adhesion in cell culture flasks

Mon Aug 08 11:43:11 CST 2022

Cell culture flasks are mostly used in the culture of adherent cells, which must adhere to the surface of a support in order to grow. So what is the mutual attraction between adherent cells and the surface of the support, and what is the principle of adherence?

Cell culture flasks

FuDau T25 Cell Culture Flasks

Cell attachment refers to the process by which anchorage-dependent cells attach and spread on a culture surface. Whether the cells can attach to the culture surface depends on the characteristics of the cells themselves, the contact probability between the cells and the culture surface, and the compatibility between the cells and the culture surface, which is related to the chemical and physical properties of the surface. nature related. The rate of cell attachment is also related to the chemical and physical properties of the culture surface, especially the charge density on the culture surface. Cryolysin and fibronectin in serum can bridge the culture surface and cells, which is beneficial to accelerate the rate of cell attachment. In addition to the above factors, the spreading of cells on the culture surface is also related to the surface condition, especially the smoothness.

Cell culture flasks

   FuDau T75 Cell Culture Flasks

Most mammalian cells grow in vivo and in vitro by attaching to certain substrates, which can be other cells, collagen, plastics, etc. in vitro. Cells first secrete extracellular matrix, which adhere to the surface of cell culture flasks. Cells then bind to these extracellular matrices through adhesion factors expressed on their surface.

In addition, in order to better promote cell adhesion, the growth surface of the cell culture flask will be specially treated to introduce hydrophilic groups to facilitate the growth of adherent cells.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.