In the process of culturing cells, changing the medium is a very routine operation, which is mainly used to remove various metabolic wastes generated during cell growth, supplement fresh culture medium, and promote cell growth. So, how to change the solution in the cell culture flasks?
Preparation: Alcohol spray can with 75% medical disinfection alcohol, PBS buffer, serum-containing medium, pasteurized pipette, pipette, waste liquid tank.
1. Put the equipment into the ultra-clean table and turn on the ultraviolet irradiation to sterilize. If the cultured cells are sensitive to temperature, you can put the serum-containing culture medium bottle in the 37℃ water bath to make the culture medium temperature 37℃, and then transfer Go to the ultra-clean bench for UV sterilization. In addition, the cell culture room also needs UV irradiation for about half an hour.
2. Take out the cell culture flasks from the incubator, spray 75% alcohol on the surface of the bottle with an alcohol spray can for disinfection, transfer to the ultra-clean table, open the lid, gently suck out the old culture medium, use a pipette to add fresh serum-containing medium, Be careful not to add the culture medium from the adherent surface of the cells, but from the side wall, gently, so as not to wash off the cells.
3. After adding the culture medium, close the lid and mark the bottle to indicate the time for changing the medium, cell type, operator and other information.
4. Before putting it into the incubator, spray the surface of the bottle with alcohol again, and then remember to tidy up the operating table, wipe the surface of the ultra-clean table with alcohol, and clean up waste liquid and garbage.
When changing the liquid, pay attention to sterility throughout the process. Operators should wear sterile clothes, sterile gloves and masks, and be careful when touching the mouth of the cell culture flasks with their hands to avoid contamination.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.