How to Prevent Mycoplasma Contamination in Cell Culture Flasks

Fri Jul 22 16:23:04 CST 2022

Mycoplasma contamination of cells is a very common problem. Mycoplasma contamination is not easy to find. After cells are contaminated by mycoplasma, a symbiotic system will be formed, resulting in the continuous expansion of the contamination, which in turn affects the growth of cells. So, how can we prevent mycoplasma contamination in cell culture flasks?

cell culture flasks

FuDau T25 Cell Culture Flasks

When cells are contaminated by mycoplasma, the expression of DNA, RNA and protein in cells changes, but the growth rate of cells generally does not have a significant impact. With the further expansion of pollution, the metabolic function of cells will be reduced, which will affect cell culture. Results of the experiment. The causes of mycoplasma contamination in cells in cell culture flasks may be cross-contamination between cells, operator's mouth and skin, working environment or experimental equipment contamination, culture medium contamination, etc. Mycoplasma contamination is difficult to find, so prevention is the key.

Start by adhering to good cell culture practices and culturing only one cell line at a time to prevent cross-contamination between cells. Secondly, strictly abide by the laboratory aseptic operation procedures, and do a good job of personal hygiene and environmental sterilization. Third, it is necessary to ensure that the cell culture medium, serum, reagents, cell culture flasks and other consumables used are sterile. Finally, an appropriate amount of antibiotics can also be added to the medium to prevent the occurrence of mycoplasma contamination.

cell culture flasks

FuDau T75 Cell Culture Flasks

In a word, mycoplasma contamination is a contamination source that is not easy to be found, but its impact on the cells in the cell culture flask is also very serious. Taking various precautions and strictly following aseptic operations can reduce the probability of contamination.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.