Basic conditions for culturing cells in cell culture flasks - suitable osmotic pressure

Sat Oct 08 14:25:58 CST 2022

The cell culture flask is a type of container that is widely used when culturing cells. Its specifications range from 25cm2 to 225cm2 to meet the needs of cell culture of different scales. Appropriate osmotic pressure is a basic condition when culturing cells.

Cells in hypertonic or hypotonic solutions can shrink or swell and rupture immediately. Therefore, osmotic pressure is one of the important conditions for culturing cells in cell culture flasks. The maintenance of osmotic pressure of mammalian and other animal tissue cells in vitro is mainly related to NaCl, but the relationship of osmotic pressure of other dielectrics cannot be ignored. The osmotic pressure is proportional to the number of molecules and ions of the solute per unit volume of solvent. For this reason, it is very important to control the ion balance in the culture medium in a certain proportion and maintain normal osmotic pressure. This is not only to maintain cell tension, but also to regulate the metabolism of cells.

cell culture flask

  FuDau T25 Cell Culture Flasks

Because extracellular ion transport and ion concentration alter the transport of other nutrients, it directly affects the basic synthesis system of cells. The ideal osmotic pressure varies with the type and race of cells. The osmotic pressure of human plasma is 290 mmol/L, which is considered to be the ideal osmotic pressure for culturing human cells in vitro. The osmotic pressure of mammalian cells is generally 290 to 300 mmol/L. Human embryonic lung fibroblasts are 250-325 mmol/L, and mice are about 310 mmol/L. In practical applications, the osmotic pressure of 260-320 mmol/L is suitable for most cells.

cell culture flask

FuDau T225 Cell Culture Flasks

Of course, in addition to the appropriate osmotic pressure, the use of cell culture flasks to cultivate cells also requires conditions such as gas environment and pH, appropriate temperature, and sterile environment, which are all necessary conditions for cell growth.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.