Application of cell culture flasks in the passage of mouse fibroblasts

Tue Nov 16 11:53:17 CST 2021

Cell culture flasks are a type of cell culture consumables that are widely used in the market, and are mainly suitable for adherent cell culture. Among them, this consumable is also used in the passage of mouse fibroblasts.

Mouse fibroblasts are special cells used for non-medical purposes such as scientific research or industrial applications. Fibroblasts have vigorous functions and weak basophilic cytoplasm. They have obvious protein synthesis and secretion activities. Under certain conditions, they Can achieve mutual transformation with fiber cells. After receiving the fibroblasts, they need to be stabilized in the incubator for about 4 hours and then cultured or passaged according to the cell density, changing the medium. The passaging operation follows the following steps:

1. Aspirate the medium in the original cell culture flask, rinse the cells twice with PBS buffer, and add 1~2 ml of 0.25% EDTA for trypsin digestion (pay attention to the digestion time, usually controlled within 1~2min).

 Application of cell culture flasks in the passage of mouse fibroblasts

2. Observe the digestion under the microscope. When the cell edge shrinks and adheres to the wall (digestion is not recommended until the cells float), remove the trypsin, add 6~8ml of complete medium, gently blow the cell layer, and try to blow off and blown away the cell layer.

3. Take out part of the cell suspension and transfer it to a new cell culture flask, add appropriate complete medium, and cultivate in an incubator.

4. Pay attention to the change of the pH value of the medium, change the medium regularly, and repeat the subculture or freeze storage when the cell density reaches 70-80%.

The main role of cell culture flasks in cell passaging is to store cells and provide an adherent growth environment for cells. It should be noted that if the cell wall is broken or leaking after receiving the cells, it means that the cells have been contaminated, and the supplier should be contacted in time.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.