Application of cell culture flasks in culturing Sf9 cells

Wed Nov 03 16:49:22 CST 2021

Sf9 cells are Spodoptera frugiperda cells, which are usually used to produce various medicinal proteins and viruses, or for basic research. When culturing sf9 cells, the commonly used cell culture consumables are cell culture flasks.

Sf9 cells are derived from the Insect Pathology Laboratory of the United States Department of Agriculture and are derived from the IPLBSF-21 cell line. The cells are regular with particles and can be cultured in suspension or adherent culture. This cell has a high expression level (especially intracellular proteins), has a faster growth rate, effective protein folding, glycosylation is similar to mammalian cells, and relatively easy enzymatic deglycosylation (for protein structure determination) Favorable), is widely used in the expression of V LPs.

Application of cell culture flasks in culturing Sf9 cells

Sf-9 cells are semi-adherent cell lines. In the medium containing 10% fetal calf serum, most of the cells usually grow adherently, and a small part is suspended. Grow in a 27°C cell incubator without CO2. Usually it can be sub-flask for passage when it grows to 80-95% density. Trypsin digestion is not required during passage. By gently pipetting the adherent cells 5-10 times with a 1 mL pipette, the cells can be diluted and passaged after they are not adherent. The common specifications of cell culture flasks include 25cm2, 75cm2, 175cm2, 225cm2, etc. The surface treated with TC is suitable for adherent cell culture, and the surface without TC treatment is suitable for suspension cell culture, which can meet the growth and culture needs of sf9 cells.

When performing cell passaging operations, it is recommended not to shake the cell culture flasks vigorously to avoid cell damage and affect the cell culture process.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.