English
Cellulae culturae lenticulaehabent collum rectum, torticollum, collum angulum, triangulum, rectangulum, etc. secundum diversas figuras. Specificationes includunt 25ml, 75ml, 175ml et 250ml. Minores magnitudines plerumque adhibentur ad cellam transeuntem, conservationem cellularum, et provisio cellularum experimentis, etc. usus.
Post cellulas crescunt in densum monolayum in cella cultura lenticulae, plerumque sunt. saturatur. Ut cellulae crescant et numerum cellularum augeant, subculturae debent. Subcultura est etiam modus conservandi species cellularum. Eodem tempore processus necessarius est ad varia experimenta utens cellulis excultis. Cellulae suspensae directe separari possunt, cellulae autem adhaerentes prius concoquendae sunt quam separari possunt. Gradus specifici sunt haec:
1. Medium culturae originalem abiicias in cella culturam ampullam plenam cellulis.
2. Solutio pancreatina 0.5-1ml 0.25% adde, ita ut cellulae in solutione utrem immergantur.
3. Flexilis spissamentum in utre pone et sub microscopio inverso pone ad cellulas observandas. Temporis labente, cellulae primae adhaerentes paulatim rotundis tendebant, et pancreatinum cum cellulae nondum delapsae erant deiectae sunt, et 10 ml culturae mediae ad digestionem terminandam additae sunt. Observatio digestionis etiam nudo oculo fieri potest. Ubi in fundo utre albo et tenuis orichalco videtur, concoctio terminari potest. Fere, digestio temporis ad cubiculi temperiem fere 1-3 minuta est.
4. Utere pipettis ad cellulas adherentes in suspensione sufflandas, eas in duos ad tres utres divide, solutionem culturae obturaculum cum spissamento iuvantis, et pone ad 37°C perdurandi culturam. Proximo die incrementum observa.
Haec applicatio ampullas culturae cellularum in cellam transitum est. Praeparatio solutionis modus digestionis est hoc modo: appende trypsin 0.25g (activitatem 1:250), adde 100ml Ca2+, Mg2+ liberam solutionem Hank solvendi, sparge sterilitatem per filtration, copia ad 4°C, et ad temperaturam redi ad 37 °F ante usum. Addi etiam potest EDTA solutioni pancreatinae ut ultimam attentionem recipiat 0.02%.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.