Kusho ukuthini ukuthi amaflaski e-cell culture awanayo i-pyrogen?

Wed May 18 15:46:36 CST 2022

Amaflaski e-Cell Cultureziwuhlobo lwezinto ezisetshenziswayo ezivame ukusetshenziswa enqubweni yesiko lamaseli. Iseli ukukhula kunezidingo eziphakeme zemvelo, futhi ukukhethwa kwezinto ezisetshenziswayo kufanele futhi kuhlangabezane nezidingo ze-pyrogen-free. Khona-ke, kusho ukuthini i-pyrogen-free?

Kunezinhlobo ezimbili zama-pyrogens. Enye i-endogenous pyrogen, esebenza esikhungweni sokulawula izinga lokushisa lomzimba le-hypothalamus futhi iyiphrotheni engakhuphula izinga lokushisa lomzimba. Enye i-exogenous pyrogen, ebhekisela ezintweni zangaphandle ezingaba imbangela yomkhuhlane. Ngokuvamile isebenza ngokuyenga ama-pyrogens angapheli. Lokhu esivame ukukubiza ngokuthi izinto ezisetshenziswayo ezingenayo i-pyrogen njengamaflaski e-cell culture kubhekisela kuma-pyrogens angaphandle.

What does it mean that the cell culture flasks are pyrogen-free?

Ama-Exogenous pyrogens ikakhulukazi ama-bacterial pyrogens, angama-metabolites amagciwane athile, izidumbu zebhaktheriya kanye nama-endotoxins. I-Gram-negative bacilli inamandla amakhulu abangela ukushisa, ilandelwa i-gram-positive bacilli. I-Gram-positive cocci ibuthakathaka. Isikhunta, imvubelo, ngisho namagciwane angakhiqiza ama-pyrogens. I-pyrogen ngokuvamile iyinkimbinkimbi eyakhiwe inhlanganisela ye-phospholipid polyol namaprotheni. I-Phospholipid polyol iyisikhungo esisebenzayo se-complex futhi inomphumela onamandla we-pyrogenic. Ukwakheka kwayo kwamakhemikhali kuyahlukahluka ngezinhlobo ezahlukene. Isisindo samangqamuzana si-5 × 104 kuya ku-5 × 105, futhi lapho isisindo samangqamuzana siseduze, umphumela wokushisa unamandla. Enqubweni ye-cell culture, uma okudlekayo kuqukethe ama-pyrogens, kuzoba nomthelela ngqo ekukhuleni nasekukhiqizeni kabusha amaseli, ngakho-ke lezi zinto ezidliwayo kufanele zenzelwe ukwelashwa okukhethekile ukuze kukhishwe ama-endotoxin angaphandle okungenzeka futhi kuqinisekiswe ukukhula okuvamile kwamaseli.

Ngaphezu kwalokho amaflask e-cell culture, izinto ezisetshenziswayo ezifana nama-micropipettes namathiphu afanayo asetshenziswa kusiko lweseli zidinga ukwelashwa okukhethekile ukuze kuqedwe ama-pyrogens ngaphambi kokuthi asetshenziswe.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.