Isingeniso sezimpawu zefekthri yeseli

Wed May 18 15:44:09 CST 2022

Isiko lamaseli alihlukaniseki ezintweni ezisetshenziswayo zamaseli. Njengethuluzi lokukhiqiza elifanele isiko lamaseli amakhulu, izimboni zamaseli ziye zasetshenziswa ngokwandayo ekukhiqizeni nasekulungiseni imithi yokugoma neminye imikhiqizo yebhayoloji.

I fektri yeseli yakhiwe ungqimba olulodwa noma ngaphezulu. Ngokwesilinganiso se-cell culture, imboni yeseli ehambisana nendawo yesiko ingakhethwa. Ukwengeza, imboni yeselula nayo inezici ezilandelayo:

1. Ngemuva kokuthi imboni yeseli ingene oketshezini futhi umlomo webhodlela wamukela umklamo womlomo obanzi, ukugcwaliswa nokuvuna uketshezi kushesha kakhulu, futhi akulula ukukhiqiza ama-bubbles. Ngesikhathi esifanayo, idizayini yomlomo omkhulu ivumela kakhulu ukushintshaniswa kwegesi futhi ifanele isiko lamaseli asondelene kakhulu.

2. Imboni yamaseli ejwayelekile ifakwe isembozo esiphefumulayo esiyi-0.2μm kanye nesembozo sokuvala, esingasetshenziswa ezindaweni ezihlukene zamasiko. Isembozo esiphefumulayo esiyinyumba sisetshenziswa endaweni ye-CO2, futhi isembozo esivaliwe singasetshenziselwa ama-incubator ajwayelekile kanye nezindlu ezibamba ukushisa ezingenayo i-carbon dioxide. Ukwengeza, isivalo sokudlulisa uketshezi singakhethwa ukuze kube lula ukudluliswa kwe-aseptic koketshezi, futhi isivalo sebhodlela esifanele ukudluliswa koketshezi singenziwa ngokwezifiso ngokuya ngezidingo zenqubo yekhasimende.

Introduction to the characteristics of the cell factory

3. I-membrane ephefumulayo yekepisi yebhodlela izoklanywa ukuthi ibe yi-hydrophobic, engeke ithinte umphumela wokuqina komoya kanye nokuphefumula kolwelwesi oluphefumulayo ngemva kokuthintana noketshezi.

4. Inqubo yokuhlanganisa iglue engenisiwe yamukelwa phakathi kwezingqimba ezihlukahlukene zefekthri yamaseli, engakwazi ukumelana nokucindezela kwe-1.5 PSI ukuze kuqinisekiswe ukuthi umkhiqizo awuvuzi phakathi nayo yonke inqubo yokutshala.

I-fektri yamaseli ifanele izinhlobonhlobo. yamasiko amaseli anamathelayo, okuhlanganisa amaseli e-Vero, amaseli e-HEK 293, amaseli e-CAR-T, ama-MRC5, amaseli e-CEF, ama-porcine alveolar macrophages, amaseli e-myeloma, amaseli e-DF-1, amaseli e-ST, amaseli e-PK15, amaseli Adherent afana namaseli e-Marc145. Iphinde ifanele isiko elimile lamaseli amisiwe njengamaseli e-CHO, amaseli ezinambuzane, amaseli e-BHK21 namaseli e-MDCK.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.