English
I-Subculture ibhekisela enqubweni yokuhlukanisa isiko libe izingxenye ezincane, ukuphinda kufakwe omunye umkhumbi wamasiko (ibhodlela), bese uhlakula. Amaflask empucuko yeseli yizinto ezisetshenziswayo ezisetshenziswa kakhulu uma kutshalwa amaseli. Ngakho-ke, kanjani ukuhlanganisa amaseli kufulaski?
Ngokujwayelekile, amaseli kumaflask e-cellculture akhula abe ungqimba owodwa ominyene futhi agcwale ngokuyisisekelo. Ukuze amaseli akwazi ukuqhubeka nokuzala futhi andise inani lamaseli, kuyadingeka ukwenza imisebenzi yokudlula. Amaseli amisiwe angahlukaniswa ngokuqondile, kuyilapho amaseli anamathelayo adinga ukugaywa ngaphambi kokuba ahlukaniswe. Ngokuvamile, ama-proteolytic enzymes (afana ne-trypsin) asetshenziselwa ukugaya amangqamuzana anamathelayo abe amaseli awodwa. I-Ethylenediaminetetraacetic acid (EDTA) nayo ingangezwa ukuthuthukisa ukugaya. Qaphela amaseli kuze kube yilapho amaseli eba yindilinga futhi aqhamuke odongeni lwamabhodlela. Le nqubo ngokuvamile ithatha imizuzu engu-5-15. Ukuze ugweme ukunqwabelana kwamangqamuzana, ungathengi ama-flask e-culture cell ngesikhathi senqubo yokugaya amaseli.
Kumaseli okunzima ukuwagayeka, ungakwazi ukwengeza isixazululo sokugaya i-trypsin EDTA futhi ubeke amaseli ku-37°C ukuze lula ukugaya. Ngemva kokuba amaseli ehlukanisiwe odongeni lwamabhodlela, ngokushesha engeza i-medium ephelele equkethe i-serum ukumisa ukugaya. Amaprotheni athile ku-serum angavimbela ama-pancreas. Umsebenzi wama-enzyme. Ngokuvamile, i-centrifugation ayidingekile. Uma amaseli edinga i-serum-free noma i-low-serum medium, i-centrifuge ku-125000g imizuzu emi-5, bese ulahla okuphakathi okungasaqhubeki, engeza okuphakathi okusha okufanelekile futhi ngobumnene uhlanganise amaseli ngaphambi kwe-subculture.
Isilinganiso sokudlula kweseli ngokuvamile singu-1:2 -1:20, kuye ngohlobo lweseli. Enqubweni yokudlula, i-trypsin izolimaza ulwelwesi lweseli lwamaseli athile. Ungasebenzisa i-cell scraper ukuze ukhuhle amaseli ngobumnene, wengeze inani elifanele le-pipette ephakathi nendawo ukuze uhlanganise amaseli, futhi uwadlulisele kuflask yesiko elisha.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.