Uwenza kanjani amaflask e-cell culture angabi nawo i-DNA ne-RNase

Wed May 18 15:43:34 CST 2022

I-cell culture iwuhlobo lobuchwepheshe bokusebenza kwe-aseptic, oludinga ukuthi indawo yokusebenza nezimo kufanele zingabi namagciwane futhi zingathintwa ezinye izinto eziyingozi. Amaflaski e-Cell Culture imvamisa ukusetshenziswa ekuhlolweni kweseli. Ayikho i-DNA ne-RNase izimfuneko eziyisisekelo zezinto ezisetshenziswayo. Ngakho-ke amaflaski e-cellculture angakwazi kanjani ukuba i-DNA- kanye ne-RNase?

Igumbi le-cell culture kanye nomgomo wokuklama ukuvikela ukungcoliswa kwamagciwane kanye nezici eziyingozi, futhi kudinga indawo yokusebenza ehlanzekile, umoya ohlanzekile, ukoma futhi akukho ntuthu. Ama-workshops okukhiqiza ama-flasks we-cell culture kanye nezinye izinto ezidlekayo zonke zingama-workshops e-GMP angu-100,000, kanye nemihlangano yokucobelelana ngolwazi yokukhiqiza yezidakamizwa zabantu nazo zingamazinga ayi-100,000. Ngakho-ke, imikhiqizo ekhiqizwa ngaphansi kwezimo ezinjalo ngokuvamile ayinayo i-DNA ne-RNA enzyme. Futhi ngemva kokuphuma komkhiqizo oqediwe, uzosebenzisa nokuhlolwa kokucekelwa phansi kwe-DNA kanye ne-RNA ukuhlola umkhiqizo, futhi yimikhiqizo efanelekayo kuphela engaphuma embonini. Izinto ezisetshenziswayo kanye nezitsha ezisetshenziswa kusiko lamaseli kufanele zifakwe ngokuzenzakalelayo ngesikhathi, futhi kufanele ziphinde zifakwe inzalo uma zingasetshenziswa isikhathi esingaphezu kweviki ngemva kokuvalwa inzalo. I-medium ephelele elungisiwe, i-pancreatin, i-PBS, njll. isetshenziswa kangcono lingakapheli isonto. Ngesikhathi esifanayo, hlola isimo seseli nsuku zonke. Egunjini lamaseli, kufanele unciphise ukuhamba futhi ukhulume kancane ngangokunokwenzeka. Uma ukhwehlela noma uthimula, qiniseka ukuthi ufulathela itafula elihlanzeke kakhulu. Gcina ukuthuthwa kwendle kwelabhorethri futhi ubeke isisekelo sempumelelo yokuhlolwa kweseli.

How to make cell culture flasks free of DNA and RNase

Qualified cell culture flasks are a key factor to ensure cell growth. Consumables and utensils used in cell culture should be autoclaved in time, and they should be re-sterilized if they are not used for more than a week after sterilization. The prepared complete medium, pancreatin, PBS, etc. are best used up within a week. At the same time, check the cell status every day. In the cell room, you should reduce walking and talk as little as possible. If you cough or sneeze, be sure to turn your back to the ultra-clean table. Keep the laboratory's environmental sanitation and lay the foundation for the success of the cell experiment.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.