English
I-cell culture isho indlela yokulingisa indawo yangaphakathi ku-vitro ukuze iphile, ikhule, ikhiqize futhi igcine ukwakheka nokusebenza kwayo okuyinhloko. Enqubweni yesiko lamaseli kusetshenziswa amaflask e-cell culture, kuzohlangatshezwana nezinkinga ezahlukahlukene. I-cell clumping wuhlobo oluvamile uma kuqhathaniswa. Uma unaka kakhulu isigaba sokuqala se-cell culture, lesi simo singagwenywa.
1. Ukuba khona kwe-calcium ne-magnesium ion endaweni ye-culture kungaholela kalula ekuhlanganiseni kwamangqamuzana, ngakho-ke lapho ugeza amangqamuzana, qaphela ukusebenzisa isisombululo sikasawoti esingenaso i-calcium-magnesium.
2. Kumaseli anamathele kakhulu, angagaywa ngamaqoqo amaningi, anganciphisa kakhulu ukulimala kwe-trypsin kumaseli, aqinisekise isimo esihle samaseli, futhi agweme ukunqwabelana kwamaseli.
3. Ukuminyana okuphezulu kakhulu kwamaseli kungaholela kalula ekuqoqeni kwamaseli. Ukuminyana okungama-70% -80% kuhle, okunganciphisa kakhulu amathuba okuhlangana kwamaseli. Uma amaseli esekhulile aba amakoloni, dlulisa amaseli ngokuminyana okuphansi kuqala, bese uwagaya futhi uwadlulise ngakusasa. Ngemuva kwamavesi amathathu alandelanayo, ama-cell clumps azoba mancane futhi abe mancane. Ngemva kwamavesi ambalwa, landela indlela evamile yokugaya kanye nesikhathi. Amaseli kancane kancane asakazeka abe ngamanye.
4. Zama ukuthi ungawanyakazisi ama-flask amasiko eseli ngamandla ngesikhathi sokugaya ukudla, ukuze amangqamuzana abonakale ewa njengama-chips. Uma amaseli ewela ku-suspension, kuba nzima ukuwashaya abe yisiqephu esisodwa, ngakho-ke amaseli kufanele avulwe umoya ngaphambi kokuba awe ukuze alawule ngokuqinile izinga lokugaya.
Ukuhlakulela amaseli kuwulwazi olujulile kakhulu. Izinga lamaseli, ukwakheka kwendlela yesiko, kanye nokukhula kwamaseli kuzothinta ukukhula kwamaseli. Ukuze ugweme ukunqwabelana kweseli kokuthi amaflask e-cell culture, ungalawula kusukela kuzici ezingenhla, ukuze uqinisekise inqubekelaphambili eshelelayo yomsebenzi wosikompilo lwamaseli.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.