English
Ngokuvamile, amangqamuzana anemetabolism eqinile nokukhula ngokushesha (njengamaseli esimila) kufanele ajovwe ukuhlushwa okuphansi; kuyilapho amangqamuzana ezicubu ezivamile ekhula kancane futhi umzimba wawo ungabi namandla ngokwanele, ukuhlushwa kwe-inoculation kungase kube phezulu; Ukuze kusetshenziswe izimo eziphuthumayo, i-inoculation ingaba ngaphansi; ngezinye izikhathi ukuze kube lula ukubhekwa kwe-cell morphology, ukuhlushwa kwe-inoculation kungancishiswa ngokufanelekile. Amaseli ajwayelekile angahlwanyelwa ngokwenani lamaseli endaweni ngayinye yefekthri yeseli, aphindaphindwe ngendawo yesiko lefekthri yeseli esetshenziswayo, bese ihlukaniswa ngenani lembewu lamaseli akhulisiwe. Inani lokujova eliphakeme kakhulu noma eliphansi kakhulu lamaseli alihambisani nokuzala kwamaseli. Inani elifanelekile lokugonywa kwamaseli liyisisekelo sokuqinisekisa ukukhula okuvamile kwamaseli.ubuchwepheshe bezamasiko budlala indima ebalulekile emkhakheni we-monoclonal antibody kanye nokulungiselela umgomo. Lapho kukhiqizwa amangqamuzana, kuzothinteka izici ezihlukahlukene, phakathi kwazo ukuminyana kwe-cell inoculation kuzophinde kuthinte inqubo yamaseli. kunomthelela oqondile ekukhuleni kwamaseli. Ukuminyana okufanele kwe-inoculation kungakhuthaza ukwanda kwamaseli okungcono. Ukuminyana kwe-inoculation ephezulu kakhulu noma ephansi kakhulu akusizi ekukhuleni kwamaseli nokwanda. Indlela yokukhetha ukugxila kokujova okufanelekile kufanele kunqunywe ngokuya nge-metabolism yamangqamuzana, ukukhula nesivinini sokuzala kanye nezidingo zomsebenzi.
Ukuminyana kwamaseli kuyisici esibalulekile esithinta amaseli akhulisiwe emboni yamaseli. Kuphela ngokulawula ukuminyana okufanele lapho ukuhlola kwesiko lamaseli kungenziwa ngokushelela.
Generally speaking, cells with strong metabolism and fast growth (such as tumor cells) should be inoculated at a lower concentration; while normal tissue cells grow slowly and their metabolism is not vigorous enough, the inoculation concentration may be higher; For emergency use, the inoculation can be lower; sometimes in order to facilitate the observation of cell morphology, the inoculation concentration can also be appropriately reduced. Ordinary cells can be seeded according to the number of cells per unit area of the cell factory, multiplied by the cell factory culture area used, and then divided by the seeding ratio of cultured cells. Too high or too low inoculation amount of cells is not conducive to cell reproduction. Appropriate cell inoculation amount is the premise to ensure the normal growth of cells.
The cell seeding density is an important factor affecting the cultured cells of the cell factory. Only by controlling the appropriate density can the cell culture experiment be carried out smoothly.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.