English
Ukungcoliswa kwamaseli kusho izinto zangaphandle ezixubene endaweni yesiko lamaseli ukuze kukhiqizwe izingxenye eziyingozi futhi kubangele ukungcola kwamaseli. Kuningi ukungcola okuvamile lapho kukhiqizwa amaseli kokuthi izimboni zamaseli, phakathi kwawo ukungcoliswa kwamakhemikhali kuwuhlobo olubaluleke kakhulu noluvamile.
Amakhemikhali amaningi endaweni yesiko angabangela ukungcoliswa kwamaseli. Futhi la makhemikhali awavimbeli njalo ukukhula kwamangqamuzana. Ezinye izinto, njengamahomoni, zingakhuthaza ukukhula kwamangqamuzana ezimbonini zamangqamuzana, kodwa izinto ezingahlanzekile, imidiya, amanzi, i-serum, ama-groofactors, neziqukathi zokugcina izinto ezivuselelayo kungaba umthombo wokungcola kwamakhemikhali.
1. Izakhamzimba ezibalulekile zesiko lamaseli, njengama-amino acid, zizophinde zibe ubuthi kumaseli uma ukugxila kudlula ibanga elifanele.
2. Olayini bamaseli abahlukene banezidingo ezihlukene zeserum kanye ne-buffer ngaphansi kwezimo ezifanele zesiko, okufanele zilawulwe ngokuqinile ngesikhathi sesiko.
3. Ukungcoliswa kwamakhemikhali okuvame kakhulu i-denaturant eyinsalela noma insipho (imvamisa endaweni engaphakathi yekepisi yebhodlela) ehlala kunqubo yokuhlanza ingilazi.
4. Amanzi ukuphela kwenhlanganisela ekhukhumala lapho eqina, ngakho lesi sici kufanele sinakwe lapho kukhethwa isitsha samaseli e-cryopreserving.
5. Ukuze ugweme ukungcoliswa kwamanzi ngama-ion ensimbi, ama-organic molecule, ubuthi obungaphakathi kwamangqamuzana nezinye izinto, amanzi ahlanzekile angenawo ukungcola kufanele asetshenziswe lapho kulungiswa uketshezi nezitsha zokuhlanza.
6. I-serum yezilwane iyindlela yemvelo evame ukusetshenziswa esikweni lamaseli, kodwa i-serum iwumthombo ongaba khona wokungcoliswa kwezinto eziphilayo namakhemikhali. Ikhono elikhuthaza ukukhula kanye nemiphumela enobuthi nemiphumela engemihle ye-serum kumaseli ahlukene incike ezintweni ezifana nomsebenzi wokuhlukanisa walawa maseli, umthombo wethishu kanye nokwakheka kwendawo ephakathi. Lapho wenza uchungechunge lokuhlola, ukuze kuqinisekiswe ukuphindaphindwa kokuhlolwa, kungcono kakhulu ukusebenzisa iqoqo elifanayo le-serum.
Lokhu okungenhla ukungcoliswa kwamakhemikhali okuvamile lapho kukhuliswa amaseli ezimbonini zamaseli. Ukwengeza, kubaluleke kakhulu ukulungisa nokugcina i-culture medium nama-reagents ngendlela efanele. Izinqubo zokusebenza ezijwayelekile kufanele zithathwe ukuze kugwenywe amaphutha afana namaphutha okubala umthamo woketshezi kanye nokuxutshwa kwezinhlanganisela ezifanayo. Ukulawula lezi zici ekuhlolweni kwe-cell culture kunganciphisa amathuba okungcola futhi kusheshise inqubo yokuhlola.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.