Ukusetshenziswa kwamafekthri amaseli ekukhiqizeni ngezinga elikhulu ama-lentviral vectors

Wed May 18 15:41:12 CST 2022

I-Lentivirus iwuhlobo lwe-viral vector eguqulwe isuka ku-human immunodeficiency virus (HIV), okuwuhlobo lwe-retrovirus. Ukuhlolwa kwe-in vitro kanye nokuhlolwa kwe-vivo, i-lentivirus isiphenduke enye yezindlela ezivame ukusetshenziswa zama-vector ukuveza izakhi zofuzo zangaphandle noma i-shRNA yangaphandle, futhi isetshenziswa kabanzi futhi kabanzi. Ekukhiqizweni kwayo ngobuningi, i-fektri yamaseli iwumkhuba osetshenziswa kakhulu wamaseli.

Ama-Vector eLentiviral angakwazi ukuhlanganisa ngempumelelo izakhi zofuzo zangaphandle noma ama-shRNA angaphandle ku-chromosome yokusingatha, ukuze kuzuzwe umphumela wokuveza ngokuqhubekayo okuhlosiwe. ukulandelana. Ngokuphathelene nokutheleleka, kungangena ngempumelelo amangqamuzana e-neuronal, i-hepatocytes, i-cardiomyocytes, amangqamuzana e-tumor, amangqamuzana e-endothelial, ama-stem cells nezinye izinhlobo zamangqamuzana, ukuze kuzuzwe imiphumela emihle yokwelashwa kwezakhi zofuzo. Kwamanye amaseli okunzima ukuwadlulisela, njengamaseli ayinhloko, ama-stem cells, amaseli angahlukanisiwe, njll., ukusetshenziswa kwama-lentiviral vectors kungathuthukisa kakhulu ukusebenza kahle kokudluliselwa kofuzo oluqondiwe noma i-shRNA eqondiwe, kanye nofuzo oluqondiwe noma i-shRNA eqondiwe ingakwazi. kuhlanganiswe kuseli yokusingatha. Amathuba e-genome anda kakhulu, futhi ukuvezwa kwesikhathi eside nezinzile kofuzo oluqondiwe noma i-shRNA eqondiwe kungenziwa kalula futhi ngokushesha.

Imboni yamaseli isetshenziswa kakhulu ekukhiqizeni okunwetshiwe kwama-lentviral vectors. Ingakhuphula indawo engaphezulu yesiko lamaseli, ngaleyo ndlela ikhulise isivuno se-vector, inciphise inani lezikhathi zokudluliswa kwegciwane kanye nokushintshwa kwe-post-transfection medium, futhi yehlise izindleko zabasebenzi. Kuyinto esebenza kahle futhi elula yesiko lamaseli esebenzisekayo.

Ngaphezu kokusetshenziswa ekukhiqizweni okukhulu kwama-lentviral vectors, izimboni zamaseli zidlala indima ebalulekile ekukhiqizweni okukhulu kokwelashwa kwamaseli, ukwelapha ngezakhi zofuzo, ukwelashwa kwegciwane i-oncolytic. , kanye nemithi yokugoma.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.