English
I-imboni yeseli iyinto esebenzisekayo evame ukusetshenziselwa isiko lamaseli abambelelayo. Phakathi nokuhlolwa, sidinga ukubamba isimo sokukhula samaseli ngesikhathi sangempela futhi senze izinguquko ezifanele ngokuya ngesimo esithile. Ukusebenza kweseli kungatholwa ngendlela ye-MTT ukuze kunqunywe ukukhula kweseli.
indlela ye-MTT, eyaziwa nangokuthi indlela ye-MTT colorimetric, iyindlela yokuthola ukuphila nokukhula kwamaseli. Umgomo wokuthola ukuthi i-succinate dehydrogenase ku-mitochondria yamaseli aphilayo inganciphisa i-MTT yangaphandle ibe yi-formazan ecwebezelayo eluhlaza okwesibhakabhaka nokunsomi engancibiliki futhi ifakwe kumaseli, kuyilapho amaseli afile engenawo umsebenzi onjalo. I-Dimethyl sulfoxide (DMSO) ingancibilikisa i-formazan kumaseli, futhi inani layo lokumunca ukukhanya lingalinganiswa nge-enzyme-linked immunosorbent assay ku-570nm wavelength, engabonisa ngokungaqondile inani lamaseli aphilayo. Ngaphakathi kwebanga elithile lenombolo yeseli, inani lokwakheka kwekristalu ye-MTT lilingana nenombolo yeseli. Le ndlela isetshenziswe kakhulu ekutholeni umsebenzi wezinto ezithile eziphilayo ezisebenzayo, ukuhlolwa kwezidakamizwa ezilwa nesimila esikhulu, ukuhlolwa kwe-cytotoxicity kanye nokuhlolwa kwe-radiosensitivity yesimila. Ibonakala ngokuzwela okuphezulu nomnotho.
Ngaphambi kokuhlola ukusebenza kwamaseli embonini yamaseli, isinyathelo esibaluleke kakhulu ukwakhiwa kwe-MTT. Ngokuvamile, ukuhlushwa kwe-MTT kule ndlela kuyi-5 mg/ml. Ngakho-ke, i-0.5 g ye-MTT ingakalwa, ichithwe ku-100 ml we-phosphate buffered saline (PBS) noma i-phenol ebomvu-free medium, ihlungwe ngesihlungi esingu-0.22 μm ukususa amagciwane esixazululweni, futhi igcinwe ku-4 ° C ebumnyameni. . . Ngesikhathi sokulungiselela nokugcina, zama ukusonga isitsha nge-aluminium foil.
Lapho usebenzisa indlela ye-MTT ukukala umsebenzi weseli efektri yamaseli, kufanele kuqashelwe ukuthi i-MTT inomdlavuza, ngakho qaphela lapho uyisebenzisa. Le ndlela ingasetshenziswa kuphela ukuthola inombolo yeseli ehlobene nokusebenza okuhlobene, kodwa hhayi inombolo yeseli ephelele. Uma uhlola imiphumela nge-microplate reader, ukuze kuqinisekiswe ukuhambisana kwemiphumela yokuhlola, ukumuncwa kwe-MTT kufanele kube kububanzi obungu-0-0.7 ngangokunokwenzeka.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.