English
Doimiy innovatsiyalar va biotexnologiyaning jadal rivojlanishi bilan regenerativ tibbiyot va hujayra terapiyasi portlovchi o'sishni boshdan kechirdi va keng ko'lamli hujayra madaniyati texnologiyasi etuklikni davom ettirdi. Ular orasida hujayra fabrikasi keng miqyosli hujayra madaniyatida keng qo'llaniladigan sarf materialdir va bu hujayra madaniyati usuli odatda silkitgichning yordamini talab qiladi.
Hozirgi vaqtda keng tarqalgan keng ko'lamli ekilgan hayvonlar hujayralari CHO, Vero, MRC-5, 2BS, 293T, L929 va inson diploid hujayralarini o'z ichiga oladi. Bu hujayralar asosan eritropoetin, monoklonal antikor tayyorlash, vaktsina ishlab chiqarish va boshqa mahsulotlarda qo'llaniladi. Hayvon hujayralarining in vitro sharoitida keng ko'lamli madaniyati sun'iy ravishda o'rnatilgan harorat, namlik, pH va erigan kislorod sharoitida hujayra madaniyati konteynerlarida yuqori zichlikdagi ommaviy ishlab chiqarishni talab qiladi. makonni tejash, mehnat sarfini kamaytirish va ifloslanishdan qochish xususiyatlari va ilmiy tadqiqot muassasalari va farmatsevtika kompaniyalari tomonidan asta-sekin ma'qullanadi. Odatda, hujayra zavodlarida keng ko'lamli ishlab chiqarish uchun shakerning yordami talab qilinadi. Buning sababi shundaki, keng ko'lamli madaniyatda konteynerlarning ko'p qatlamlari mavjud. Shakerdan foydalanish suyuqlik bilan oziqlantirish, hujayralarni aralashtirish, ovqat hazm qilish va oqava suv kabi operatsiyalarni avtomatlashtirishni amalga oshirishi mumkin. va yuqori samaradorlik, og'ir qo'l operatsiyalari va inson omillari tufayli yuzaga keladigan xatolarni kamaytirish.
Bundan tashqari, silkitgichdan foydalanish ommaviy uzatishda rol o'ynashi mumkin, bu esa namuna olish va turli parametrlarni aniqlash uchun qulaydir. Shuning uchun, hujayra zavodlarini keng ko'lamli madaniyat uchun ishlatganda, kultura samaradorligini oshirish va hujayra madaniyatining silliq rivojlanishini ta'minlash uchun odatda silkituvchi ishlatiladi.
In addition, the use of a shaker can play a role in mass transfer, which is conducive to the sampling and determination of different parameters. Therefore, when using cell factories for large-scale culture, a shaker is generally used to improve the culture efficiency and ensure the smooth progress of cell culture.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.