English
Hujayra kulturasi uchun hujayra madaniyati flasks dan foydalanganda hujayra to'planishi keng tarqalgan muammo hisoblanadi. Hujayralarning to'planishi hujayralarning normal o'sishi va ko'payishiga ta'sir qiladi. Keyin, bu muammoni qanday hal qilish mumkin?
Birinchidan, hujayraning to'planishi hujayra madaniyati jarayoniga ta'sir qilishi shart emas. Hujayra aniq konturga va o'tkazuvchan hujayra tanasiga ega bo'lsa, bir xilda to'plangan bo'lsa, bu hujayraning yaxshi o'sishini isbotlaydi. Biroq, ko'p hollarda, to'plangan hujayralar loyqa konturlari, yomon yorug'lik o'tkazuvchanligi va hatto hujayra qoldiqlari bilan birga sintsitiyaga ega bo'lib, bu hujayralar qarigan yoki kasal bo'lganligini va yomon ahvolda ekanligini isbotlaydi. Hujayra to'planishi muammosini hal qilishning bir qancha usullari mavjud:
1. Agar hujayraning to'planishi o'sishga ta'sir qilmasa, hech qanday muammo yo'q, lekin hisoblash qiyinroq. Ovqat hazm qilish jarayonida hujayralar ko'pburchak shakldan dumaloq shaklga o'tishdan oldin hujayralar devordan tushib ketishi uchun qo'lingiz bilan hujayra madaniyati kolbasining yon devoriga teging (uflamaslik yaxshidir, bu esa hujayra uchun yaxshi emas. hujayralarning shakli va o'sishi). Kimga:
2. Agar siz o'lik hujayralar DNKsini sezsangiz, DNK zanjirini buzish uchun hujayra suspenziyasiga bir necha tomchi steril DNKaz (suvda erigan 1 mg / ml) qo'shing. Hujayra membranasiga jismoniy shikast yetkazmaslik uchun hujayralarni muloyimlik bilan puflang.
3. Agar hujayra bo'laklari yalang'och ko'z bilan ko'rinadigan bo'lsa, hujayralar taxminan yarim daqiqa tursin, so'ngra o'tish uchun hujayra suspenziyasining yuqori yarmini bo'laklarsiz pipetka bilan o'tkazing. Agar hujayra bo'laklari yalang'och ko'zga ko'rinmasa, hozirda alohida yaxshi ajratish usuli mavjud emas va bu hujayralar hujayra holati yaxshilangandan keyingina asta-sekin yo'q qilinishi mumkin.
Agar hujayra madaniyati kolbalaridagi hujayralar to'planib qolgandek ko'rinsa, ular yuqoridagi usullar bilan davolash mumkin. Hujayra madaniyati jarayonida hujayralarning o'sish holatini o'z vaqtida kuzatish va vaziyatga qarab tegishli tuzatishlar kiritish kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.