English
Hujayralarni kriyoprezervatsiya qilish - bu uzoq muddatli saqlash uchun hujayra metabolizmini kamaytirish uchun hujayralarni past haroratli muhitga joylashtiradigan usul. Hujayralarni himoya qilishdan tashqari, ushbu texnologiya ko'pincha ma'lum hujayralarni sotib olish, sovg'a qilish, almashtirish va tashish uchun ishlatiladi. Xo'sh, qanday qilib kriyoprezervatsiya qilingan hujayralarni hujayra madaniyati kolbalariga o'tkazamiz?
Muzlatilgan hujayralar keyingi operatsiyani o'tkazishdan oldin reanimatsiya qilinishi kerak. Hujayralarning reanimatsiyasi tez bo'lishi kerak, shuning uchun hujayralarning hayotiyligi yaxshi saqlanib qolishi mumkin. Operatsiya quyidagicha amalga oshirilishi mumkin:
1. Hujayralarni qabul qilgandan so'ng, tashqi qadoqlash to'liq yoki yo'qligini tekshiring; tashqi o'ramni oching va quruq muzda saqlangan hujayralarni oling;
2. Kriyokonservalash trubkasi shikastlangan yoki yo'qligini, tarkibi eritilgan yoki yo'qligini tekshirib ko'ring va olib tashlangan kriyokonserva trubkasini eritish uchun tezda 37 ℃ suv hammomiga qo'ying;
3. Kriyokonserva trubkasi 75% spirt bilan dezinfektsiya qilinadi va biologik xavfsizlik kabinetiga joylashtiriladi;
4. Foydalanish uchun 15ml santrifuga trubasiga 5ml toʻliq muhit qoʻshing;
5 Tayyorlangan muhitga kriyokonservatsiya trubkasidagi hujayra suspenziyasini qoʻshing va muloyimlik bilan aralashtiring;
6. 15 ml santrifüj trubkasini santrifugaga soling va 1000 rpm/min (taxminan 200 g) tezlikda 3-5 daqiqa davomida santrifüj qiling;
7. Foydalanish uchun 25 ml lik hujayra kulturasiga 5 ml to‘liq muhit qo‘shing;
8. Santrifüjlangan hujayralarni chiqarib oling, ularni spirt bilan sterilizatsiya qiling va xavfsiz shkafga qo'ying;
9. Santrifüj qilingan hujayra supernatantini sekin aspiratsiya qiling, hujayralarni qayta suspenziya qilish uchun 1 ml to'liq muhit qo'shing;
10. Tayyorlangan hujayra madaniyati kolbasiga qayta suspenziyalangan hujayralarni qo'shing va muloyimlik bilan aralashtiring;
11. Mikroskop ostida emlangan hujayralarning holati va zichligini kuzating va kulturani madaniyat uchun 37°C inkubatorga qo'ying.
Hujayra tiklanish jarayonida tez sintez hujayradan tashqari kristallarning qisqa vaqt ichida erishini ta'minlaydi. vaqt o'tishi va hujayra ichidagi qayta kristallanishni hosil qilish uchun suvning hujayralarga kirib borishiga olib keladigan sekin erishi tufayli hujayralarga zarar yetkazmaslik. hujayra madaniyati kolbalari va boshqa sarf materiallari aseptik bo'lishi kerak.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.