English
hujayra madaniyati sarf materiallarining ko'p qatlamli tuzilishi bo'lib, asosan keng ko'lamli in vitro hujayra madaniyati uchun ishlatiladi. Hujayralarni etishtirishda ma'lum bir muhit talab qilinadi va gaz muhiti va pH qiymati eng muhim shartlardan biridir.Hujayra zavodi hujayralarni etishtirishda nazarda tutadigan gaz muhiti asosan karbonat angidrid va kislorod. Kislorod hujayra o'sishi, ko'payishi va turli zarur komponentlarning sintezi uchun energiya ishlab chiqarish uchun hujayraning trikarboksilik kislota aylanishida ishtirok etadi. Ba'zi hujayralar gipoksik sharoitda glikoliz orqali energiya olishlari mumkin, ammo ko'pchilik hujayralar gipoksiya ostida yashay olmaydi. Kislorod kuchlanishi odatda atmosfera sharoitidan biroz pastroqda saqlanadi va kislorodning qisman bosimi atmosferadagi kislorod miqdoridan oshsa, ba'zi hujayralar zararli bo'lishi mumkin. Ochiq kulturadan foydalanilganda (idish-tovoq yoki kultura shishasi bo'shashgan kultura yoki kultura plastinkasi madaniyati) hujayralar odatda 95% havo va 5% karbonat angidriddan iborat aralash gaz muhitiga joylashtiriladi.hujayra fabrikasi
Karbonat angidrid nafaqat uning metabolitidir. hujayralar, balki hujayra o'sishi uchun muhim komponent bo'lib, madaniyat muhitining pH darajasini saqlash bilan bog'liq. Yopiq muhitda past karbonat angidrid konsentratsiyasi bo'lsa, hujayralar o'sishi oson; odatda 1% dan past bo'lishi mumkin emas, aks holda hujayralar shikastlanadi. Agar ochiq kultura 5% karbonat angidridni o'z ichiga olgan gaz muhitiga joylashtirilsa, maqsadga muvofiqdir.
Ko'pchilik hujayralar pH 7,2 ~ 7,4 da o'sishga mos keladi, pH 6,8 dan past yoki pH 7,6 dan yuqori hujayralar uchun zararli va hatto buziladi yoki o'ladi. Turli hujayralar pH qiymati uchun turli talablarga ega. Odatda, birlamchi madaniyat hujayralari ishqoriy pH qiymatiga kislota bardoshliligiga qaraganda kamroq bardoshlidir va kislotali muhit ishqoriy muhitga qaraganda hujayra o'sishi uchun ko'proq foydalidir. Madaniy muhitning doimiy pH qiymatini saqlab turish uchun muhitga fosfat kabi bufer qo'shish usuli ko'pincha qo'llaniladi.
Hayotshunoslik sohasining jadal rivojlanishi bilan hujayra fabrikalari ko'proq qo'llaniladi. dalalar. Ammo u qaysi sohada qo'llanilishidan qat'i nazar, hujayra o'sishi uchun gaz muhiti va pH qiymati baribir hujayra o'sishi uchun muhim shartlardan biri hisoblanadi.
With the rapid development of the field of life science, cell factories are being applied to more fields. But no matter which field it is used in, the gas environment and pH value during cell culture are still one of the important conditions for cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.