English
Yuqori samaradorlik erlenmeyer silkitish flask - bu asosan sutemizuvchilar hujayralari, hasharotlar hujayralari va boshqa suspenziya hujayralarini etishtirish uchun ishlatiladigan keng tarqalgan hujayra madaniyati. Ular orasida CHO hujayra antikorlari farmatsevtikasi uning qo'llanilishi sohasining muhim jihati hisoblanadi.
CHO hujayralari birinchi marta 1957 yilda Puck laboratoriyasi tomonidan ajratilgan va 0,1 g xitoy hamsteri tuxumdon to'qimasini fermentativ hazm qilish yo'li bilan olingan. Odamlarga o'xshash murakkab post-translational modifikatsiyalari va sarumsiz va kimyoviy jihatdan aniqlangan muhitda tez va mustahkam suspenziya o'sishi tufayli CHO hujayralari ko'pincha faol biologik makromolekulyar oqsillarni ifodalash uchun ishlatiladi. Hozirgi vaqtda sotuvga chiqarilgan terapevtik oqsillarning deyarli 70% CHO hujayralari tomonidan ifodalanadi va ishlab chiqariladi va CHO hujayralari murakkab post-translatsion modifikatsiyani talab qiladigan terapevtik oqsillarni ishlab chiqarish uchun asosiy vositaga aylandi. Masalan, 2021 yil mart oyida ishga tushirilgan rekombinant yangi koronavirus vaktsinasi (CHO xujayrasi) CHO hujayralaridan ishlab chiqilgan. Printsip yangi koronavirus S oqsil retseptorlari bog‘lovchi hududi (RBD) genini xitoy hamster tuxumdoni (CHO) hujayra genlariga rekombinatsiya qilish, RBD dimerini hosil qilish uchun in vitro ifodalash va immunogenlikni yaxshilash uchun alyuminiy gidroksid adjuvantini qo‘shishdan iborat.
Ertada CHO hujayra antikorlari farmatsevtika tadqiqotlari bosqichi, yuqori samarali erlenmeyer silkituvchi flakonlar tez-tez ishlatiladigan sarf materiallari hisoblanadi. Reanimatsiya qilingan hujayralar yuqori samarali silkituvchi kolbada 2-3 kun davomida ekilganidan keyin hujayra zichligi oshadi va oziq moddalar asta-sekin iste'mol qilinadi. Bu vaqtda hujayralar kengaytirilishi kerak. Kengayish hajmini hujayra zichligiga qarab hisoblang. Reaktorda emlash kerak bo'lgan hujayralar soniga yetganda, silkitiladigan kolba kulturasini to'xtating, reaktorni kultura uchun emlang va kultura tugagandan so'ng hujayralarni yig'ib oling.
CHO hujayra antikorlari dorixonasi dasturning faqat bir jihati hisoblanadi. yuqori samaradorlik erlenmeyer kolbalarni silkiting.. Bundan tashqari, ushbu sarflanadigan material monoklonal antikorlarni tadqiq qilish va sut zavodlarida bakteriyalarni fermentatsiyalash va etishtirishda muhim rol o'ynaydi.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.