English
Rast celic ni odvisna samo od obilja hranilnih snovi, ampak mora biti tudi znotraj ustreznega območja pH vrednosti za normalno rast in razmnoževanje. Zato je treba pred uporabo cell factory za obsežno celično kulturo najprej izmeriti in prilagoditi pH vrednost medija.
Vrednost pH se nanaša na razmerje med skupnim številom vodikovih ionov v raztopini na celotno količino snovi. Na splošno je pH vrednost medija, ki je potrebna za rast celic, 7,2-7,4. Previsoka ali prenizka ne prispeva k rasti celic. Tovarna celic uporablja tekoči medij za gojenje celic, ki ga lahko izmerimo s pH metrom. pH meter je poseben instrument za merjenje pH vrednosti raztopine. Meri pH raztopine z elektrodo za izbiro pH (kot je steklena elektroda), rezultat meritve pa je lahko natančen na dve decimalni mesti.
Kalibrirati ga je treba s standardno pufrsko raztopino znanega pH (običajno pH 4~7) pred uporabo. Elektrodo pH metra speremo z destilirano vodo in nato potopimo v medij čaše, pH medija pa lahko odčitamo z skale pH metra. Vsak proizvajalec zagotavlja podrobna navodila za uporabo za vsak različen pH meter.
Če se po meritvi odstopa od pH vrednosti medija, ki ga uporablja tovarna celic, jo je treba ustrezno prilagoditi. PH meter lahko umerimo s standardno pufersko raztopino z znanim pH, nato se sestavljena elektroda in uporovni termometer damo v gojišče, ter počasi dodamo ustrezno količino klorovodikove kisline in natrijevega hidroksida s konca čaše stran od elektrodo za pripravo gojišča. Dosežite in vzdržujte želeni pH.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.