What to do with cell clusters in cell culture flasks

Tue Nov 09 11:53:17 CST 2021

Cell clumping is a common problem when using cell culture flasks for cell culture. The clumping of cells will affect the normal growth and reproduction of cells. Then, how to solve this problem?

First of all, cell clumping does not necessarily affect the cell culture process. If the cell has a clear outline and permeable cell body, clustered uniformly, it proves that the cell is growing well. However, in most cases, the clustered cells have fuzzy outlines, poor light transmission, and even syncytia, accompanied by cell debris, which proves that the cells have been senescent or diseased and are in poor condition. There are several ways to solve the problem of cell clumping:

1. If the cell clumping does not affect the growth, there is no problem, but it is more troublesome to count. During digestion, before the cells change from a polygonal shape to a round shape, tap the side wall of the cell culture flask with your hand to make the cells fall off the wall (it is best not to blow, which is not good for the shape and growth of the cells). To

2. If you feel the DNA of dead cells, add a few drops of sterile DNAse (1mg/ml dissolved in water) to the cell suspension to break the DNA strand. Blow the cells gently to prevent physical damage to the cell membrane.

What to do with cell clusters in cell culture flasks

3. If the cell clumps are visible to the naked eye, let the cells stand for about half a minute, and then pipette the upper half of the cell suspension without clumps for passage. If the cell clumps are not visible to the naked eye, there is currently no particularly good separation method, and these cells can only be slowly eliminated after the cell condition improves.

If the cells in the cell culture flasks appear clumping, they can be treated according to the above methods. During the cell culture process, it is necessary to observe the growth status of the cells in time and make corresponding adjustments according to the situation.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.