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What to do if the cells do not adhere to the wall when culturing cells in the cell factory

Thu Apr 14 11:03:47 CST 2022

Cell factories are mainly used for large-scale culture of adherent cells, such as hepatitis A vaccine, hepatitis B vaccine, chickenpox vaccine, etc. When culturing cells, sometimes we find that cells are difficult to adhere to the wall. What is the reason?

First of all, there are many reasons for the phenomenon of non-adherence when the cells are cultured in the cell factory, which can be analyzed and solved from the following aspects:

Excessive trypsin digestion: Trypsin helps cells to digest. If the digestion is excessive, the activity of cells will be greatly damaged, and cells will float. This can be controlled by shortening the digestion time or lowering the trypsin concentration.

Mycoplasma contamination: cells are sensitive to the environment. If the operator does not pay attention to hygiene, or the working environment and experimental equipment are contaminated, it will lead to cross-contamination between cells and the phenomenon of non-adherence. If mycoplasma contamination is found, promptly discard the culture.

Cellular Aging: Cellular aging is the sum of degenerative changes in cells as the organism ages, causing cells to lose their adherence if they are confluent before passage. Freshly seeded cells can be thawed and re-cultured.

In addition, if the initial concentration of inoculated cells is too low or too high, it will also affect the adherence properties of cells, and adjusting the appropriate concentration of inoculated cells can solve this problem. Before using the cell factory, operators should do a good job of disinfection of themselves and the environment to avoid various contamination affecting the cell culture process.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.