English
A PETG medium bottle is a special container used to store serum. It is transparent in appearance and square in shape, making it easy to hold. When storing serum, we often find sediments in the bottle. What are these sediments? Will it affect the quality of the serum? This article will answer these questions in detail.
Serum precipitate refers to the solid particulate matter that appears in serum during cell culture. The main components of the sediment are proteins, polysaccharides, lipids, inorganic salts, etc. The main reasons are twofold. First, the coagulation factors in the serum will be activated under certain conditions, leading to the polymerization and precipitation of fibrinogen in the serum. The second is the growth of microorganisms such as bacteria and fungi: Microorganisms such as bacteria and fungi that may be present in the serum will grow and reproduce under suitable conditions, resulting in precipitates in the serum.
If you find sediment in the PETG medium bottle, you can take the following measures:
1. Check the source of the serum: Make sure the serum is sourced from a reliable supplier and used according to the manufacturer’s recommendations.
2. Filtration treatment: You can use a filter to filter the serum to remove the sediment. Filtered serum should be tested for sterility before use.
3. Avoid repeated freezing and thawing: Repeated freezing and thawing may cause crystallization and precipitation of components in the serum. The number of freezing and thawing of serum should be minimized.
4. Use other cell culture reagents in conjunction with other cell culture reagents: Use other cell culture reagents, such as growth factors, antibiotics, etc., to improve the nutritional value and stability of serum.
5. Pay attention to storage conditions: When storing serum, attention should be paid to temperature, humidity, light and other conditions to avoid affecting its quality.
In short, although serum sediment may have a certain impact on cell culture, its impact can be minimized through reasonable handling. During the cell culture process, you should pay close attention to the quality changes of the serum in the PETG medium bottle, and take corresponding treatment measures in a timely manner to ensure the normal growth and differentiation of cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.