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PETG media bottles are special containers used for storing serum, which are transparent and square in appearance and easy to grip. When storing serum, we often find that there are sediments inside the bottles, what are these sediments? Does it affect the quality of serum? This article will answer these questions in detail.
First, recognize the serum precipitate
Serum precipitate refers to the solid particulate matter in the serum during cell culture. The composition of the precipitate is mainly proteins, polysaccharides, lipids, inorganic salts and so on. The reason for its production is mainly twofold, one is that the coagulation factors in serum are activated under certain conditions, leading to the polymerization and precipitation of fibrinogen in serum. The second is the growth of microorganisms such as bacteria and fungi: microorganisms such as bacteria and fungi that may be present in the serum will grow and multiply under suitable conditions, leading to the appearance of precipitates in the serum.
Second, treatment
In the process of cell culture, if the serum in PETG media bottle is found to have precipitates, the following measures can be taken:
1. Check the source of serum: Make sure the serum comes from a reliable supplier and is used according to the manufacturer's recommendation.
2. Filtering: The serum can be filtered using a filter to remove any precipitates. Filtered serum should be tested for sterility before use.
3. Avoid repeated freezing and thawing: Repeated freezing and thawing may lead to crystallization and precipitation of the components in the serum, and the number of times the serum is frozen and thawed should be minimized.
4. Cooperate with other cell culture reagents: Cooperate with other cell culture reagents, such as growth factors, antibiotics, etc., can improve the nutritional value and stability of serum.
5. Pay attention to the storage conditions: When storing serum, attention should be paid to the conditions of temperature, humidity and light to avoid the impact on its quality.
In conclusion, although serum precipitates may have a certain impact on cell culture, their impact can be minimized by reasonable treatment. In the process of cell culture, the quality change of serum in PETG media bottles should be closely monitored, and corresponding treatment measures should be taken in time to ensure the normal growth and differentiation of cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.