As a multi-layer cell culture container, cell factory systems are widely used in vaccine preparation, biomedicine, cell therapy and other fields. The correct operation method is the key to ensure the effect of cell culture, so we need to pay attention to the following points when culturing cells:
1. Preheating in advance: The cell factory systems are a commonly used tool for large-scale cell culture. Due to the relatively large culture area, it takes a long time to reach the set culture temperature. We can preheat the container and the required medium before the start of the experiment, which can effectively accelerate the speed of cell attachment and help improve the cell recovery rate.
2. Horizontal placement: Whether the cells are evenly distributed is also one of the factors that affect cell growth, so we must pay attention to horizontal placement when placing the culture container to avoid uneven cell distribution due to uneven surface.
3. Slow operation: Due to the multi-layer structure, air bubbles are likely to be generated during operation, which will cause the medium to flow from the upper layer to the lower layer, resulting in uneven distribution of the liquid level and affecting cell growth. Therefore, the action should be slow when operating, try to ensure that each layer of solution is evenly distributed, and avoid large-scale shaking to generate air bubbles.
4. Alcohol disinfection: We need to use alcohol for disinfection during operation. When spraying alcohol, pay attention to the spraying range to avoid spraying on the ventilating cover, which will affect the gas exchange.
To sum up, when using cell factory systems for large-scale cell culture, we must pay attention to the above four points. In addition, we must strictly follow the aseptic principle during operation to avoid introducing pollution sources.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.