The importance of shaker speed to cells in cell shaker flasks

Wed Sep 28 11:34:30 CST 2022

Erlenmeyer shake flask is a kind of cell consumable that is often used in suspension cell culture. Unlike adherent cells, suspension cells grow in suspension in culture medium. In addition to the gas environment, pH, osmotic pressure and other conditions, the speed of the shaker also affects the growth of cells.

Cell culture requires a suitable growth environment and growth temperature, which requires the selection of a suitable shaker for assistance. The constant temperature shaker is a special equipment used in conjunction with the cell shaker. When culturing cells, the shaker needs to be continuously rotated to provide gas conditions for the cells, and at the same time, it can also avoid cell death caused by cell deposition.

Erlenmeyer shake flask

          FuDau 125ml Erlenmeyer Shake Flasks 

The specifications of erlenmeyer shake flasks include 125ml, 250ml, 500ml, 1000ml, 3L, 5L, etc. The volume of medium added to the flasks of different specifications is also different, and the oxygen demand of different cells will also be different. Therefore, the rotation speed of the shaker is generally determined according to the type of cells and the capacity of the medium. If the rotation speed is too high, foam is likely to occur, and if the rotation speed is too low, the cells are easily deposited. Both of these conditions are conducive to the growth of cells. Taking BHK-21 cells as an example, the volume ratio of the medium added is generally 25%. The recommended speed of the bottle within 1L is 120 rpm, while the large-capacity shake flask adds more medium, so the speed will be different.

Erlenmeyer shake flask

            FuDau 250ml Erlenmeyer Shake Flasks

In summary, the rotation speed of the shaker will directly affect the growth of cells in the erlenmeyer shake flask, and an appropriate rotation speed is the basic condition for improving cell viability. Of course, the specific speed setting also depends on the type of cells and the volume ratio of the medium added.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.