The difference between the sealing cap and the breathable cap of the cell culture flasks

Tue Oct 26 14:51:21 CST 2021

The cell culture flasks is a kind of cell culture consumable, which plays an important role in the medium-scale cell and tissue culture in the laboratory. The bottle cap of this kind of bottle is divided into two types: a sealed cap and a breathable cap. Then the two caps are respectively, what kind of scenario is applicable and what is the difference?

The cell culture environment includes sterile, suitable temperature (37~38℃), osmotic pressure (260~320mmol/L), carbon dioxide and suitable pH (7.2~7.4). Cell culture square flasks generally require the help of incubators or greenhouses for cell culture. According to the different use environments, the lids are divided into two types: sealed lids and breathable lids.

The difference between the sealing cap and the breathable cap of the cell culture flasks

Sealing cap: This kind of cap is completely sealed. There are no vents on the cap. It is mainly used under carbon dioxide-free conditions such as incubators and greenhouses. It has good airtightness and can prevent the invasion of external bacteria and promote cell reproduction. Create a good growth environment.

Breathable cover: This kind of cover is equipped with vent holes, these holes can let the carbon dioxide in the environment enter the cell culture square flask, and create suitable growth conditions for cell growth. There is a sterile breathable membrane on the top of the bottle cap, which has good waterproof and breathable functions. The liquid in the cell culture square bottle does not affect the microbial barrier and breathability of the breathable membrane after contact, which can ensure a good growth state of cells.

The two caps of the cell culture flasks meet the needs of different culture environments for cell growth. When choosing the cell culture flasks, you should choose a suitable cap according to the specific cell culture environment to avoid affecting cell growth.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.