In cell culture experiments, attention to detail is critical, and any oversight can have an impact on the results, especially when using multi-layer culture vessels such as cell factories. Today we will talk about the precautions when using cell factories to cultivate cells.
Cell Culture Considerations:
1. All operations must be carried out in strict accordance with the requirements of aseptic operation.
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2. Preheating in advance: The larger the culture vessel, the longer it takes to reach the set culture temperature, so preheating the cell factory and medium to the culture temperature before the start of the experiment can speed up cell attachment and significantly improve cell recovery Rate.
3. Please operate slowly to avoid large shaking to generate air bubbles. Air bubbles can cause the medium to flow from the upper layer to the lower layer, resulting in uneven distribution of the medium.
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4. Avoid spraying alcohol or disinfectant on the vent cover. Alcohol or disinfectant may wet the hydrophobic filter membrane, resulting in non-breathing, affecting gas exchange or causing the pressure to be unbalanced during operation.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.