When we use some cell culture consumables, we always encounter the problem of cell passage. Today, Fudau will briefly share with you how to use high-efficiency shake flasks for cell passage. When we use high-efficiency shake flasks for cell passage, there are two methods for you to choose from, such as collecting cells by centrifugation and then passage, or direct passage.
Centrifugal passage method:
(1) Transfer the cells in the high-efficiency shake flask together with the culture medium to a centrifuge tube for centrifugation.
3L Erlenmeyer Shake Flasks
(2) Discard the supernatant, add new culture medium to the centrifuge tube, and pipette to form a cell suspension.
(3) Count and inoculate in new culture flasks respectively.
If direct passage is adopted, let the suspended cells slowly settle on the bottom of the high-efficiency shake flask, suck off 1/2~2/3 of the supernatant, and then pipette to form a cell suspension before passage.
5L Erlenmeyer Shake Flasks
What we need to pay attention to during the operation is that the trypsin should be pre-warmed, and the temperature is about 37°C. The centrifugation speed should be appropriate. If the speed is too low, the cells cannot be separated effectively. If the centrifugation speed is too high and the time is too long, the cells will be squeezed, causing damage or even death. The cells should be observed regularly, and if contamination is found, it should be dealt with in time.
This is how Fudau shared with you how to use high-efficiency shake flasks for cell passage. I hope it will be helpful to you.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.