How to detect mycoplasma contamination when culturing cells in the cell factory

Thu Dec 01 10:15:30 CST 2022

Fudau has briefly described the characteristics and manifestations of mycoplasma contamination in the cell factory before, because if the cells in the cell factory are contaminated by mycoplasma, the culture medium in the cell factory will not appear turbid in general, then we can through these four detection methods:

1. Detection of mycoplasma contamination by phase contrast microenvironment

Put the cells suspected of being contaminated by mycoplasma in the cell factory on the cover glass according to the species, take them out after standing for a day, and use a phase contrast oil lens to assist in observing the state of the cells. I have briefly introduced the shape of mycoplasma before. If the cells in the cell factory are contaminated Mycoplasma pollution, then after observation, it will be found that there are some dark tiny particles doing irregular movements on the cell surface and directly between cells.

Cell Factory5-Layers-Cell-Factory

2. Fluorescent staining

The DNA fluorescent staining method is based on the principle that the fluorescent dye bibendazole Hoechst33258 can bind to the A-T base-rich region in the DNA of mycoplasma. After staining the cells contaminated by mycoplasma, many cells of uniform size can be seen outside the nucleus and around the cell. Fluorescent dots are mycoplasma DNA rich in A-T base regions.

3. Electron microscope inspection

The scanning electron microscope method is simple and fast. Generally, the cells in the cell factory are cultured for 48 to 72 hours. Before the cells are close to confluence, the cells are digested with trypsin to make a cell suspension, and then fixed, embedded, and sliced ​​before observation can be performed.

Cell Factory10 Layers Cell Factory

4. Methods such as DNA molecular text inspection or mycoplasma culture

The above are the four methods introduced to you to detect mycoplasma contamination when culturing cells in cell factories. I hope they can help you.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.