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In biomedical research, Erlenmeyer Shake Flasks are ideal tools for cell culture due to their unique conical design. It not only provides sufficient oxygen and nutrients to the cells, but also effectively prevents contamination and ensures a sterile culture environment. However, to fully utilize the advantages of Erlenmeyer Shake Flasks, the following key points must be noted when using them:
1. Ensure aseptic operation
Before using Erlenmeyer Shake Flasks, it is important to make sure that the bottles are in a sterile state. Any tiny contamination may affect cell growth or even lead to experiment failure. It is recommended to use sterilized products and perform cell inoculation in the aseptic operating table.
2. Correctly set up the culture conditions
Place the Erlenmeyer Shake Flasks in a constant temperature shaker or incubator and set the appropriate temperature, humidity and rotation speed. By adjusting the rotational speed and amplitude of the shaker, it can simulate the movement environment of the cells in the body and promote the uniform distribution of nutrients and gas exchange. Observe the cell growth regularly and adjust the culture conditions in time.
3. Succession operation
When the number of cells reaches a certain density, it is necessary to carry out passaging operation. Be careful to operate gently to avoid damaging the cells. Use appropriate amount of trypsin or other digestive reagents to ensure that the cells are evenly dislodged from the wall of the flask.
4. Avoid violent shaking
Although Erlenmeyer Shake Flasks are suitable for use in a shaker, violent shaking may cause mechanical damage to the cells, affecting their growth and propagation. It is recommended to choose the appropriate speed (usually 80-120 rpm) according to the cell type and avoid sudden acceleration or deceleration.
5. Change the culture medium regularly
According to the cell growth rate and experimental needs, change the culture medium regularly and replenish fresh reagents. Metabolic wastes in old media may inhibit cell growth, so timely replacement is key to ensuring cell health.
Why choose Erlenmeyer Shake Flasks?
Efficient Oxygen Supply: The conical design promotes gas exchange and meets the cell's need for oxygen.
Anti-Contamination: Closed structure effectively prevents the entry of external contaminants.
Wide range of application: suitable for suspension cells, adherent cells and other culture methods.
Erlenmeyer Shake Flasks are indispensable tools for cell culture, but their efficient use depends on correct operation and careful maintenance. By following the above precautions, you will be able to fully utilize the advantages of Erlenmeyer Shake Flasks to ensure the smooth running of your experiments. If you are looking for high quality Erlenmeyer Shake Flasks, please contact us for more product information and technical support!
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.