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In biomedical research, the Erlenmeyer Shake Flask, with its distinctive conical design, is an ideal tool for cell culture applications. It provides sufficient oxygen and nutrients for cells while effectively minimizing contamination risks, ensuring a sterile culture environment.
However, to fully maximize the advantages of an Erlenmeyer Shake Flask, the following key considerations should be observed during use:
1. Ensure Aseptic Operation
Before using an Erlenmeyer Shake Flask, confirm that the flask is sterile. Even minor contamination can significantly affect cell growth and may lead to experimental failure.
It is recommended to use pre-sterilized products and perform all inoculation procedures inside a biological safety cabinet or laminar flow hood to maintain aseptic conditions.
2. Properly Set Culture Conditions
Place the Erlenmeyer Shake Flask in a temperature-controlled incubator shaker or incubator. Set appropriate parameters for temperature, humidity, and agitation speed.
By adjusting the shaker speed and orbital diameter, you can simulate physiological motion conditions, promoting:
Uniform nutrient distribution
Enhanced gas exchange
Improved cell growth performance
Regularly monitor cell morphology and growth status, and adjust culture parameters as needed.
3. Subculture (Passaging) Procedures
When cell density reaches the appropriate level, subculture is required.
During passaging:
Handle cells gently to avoid mechanical damage
Use an appropriate amount of trypsin or other dissociation reagents
Ensure cells detach evenly from the flask surface
Proper technique helps maintain high cell viability and consistent culture performance.
4. Avoid Excessive Agitation
Although Erlenmeyer Shake Flasks are designed for use on orbital shakers, excessive agitation can cause mechanical stress and negatively impact cell growth.
It is generally recommended to select an appropriate shaking speed based on the specific cell type (typically 80–120 rpm) and avoid sudden acceleration or deceleration.
5. Replace Culture Medium Regularly
Depending on cell growth rate and experimental requirements, replace the culture medium at appropriate intervals and supplement with fresh reagents as needed.
Accumulated metabolic waste products in spent medium may inhibit cell growth. Timely medium replacement is essential for maintaining optimal cell health and productivity.
Why Choose an Erlenmeyer Shake Flask?
Efficient Oxygen Transfer
The conical design enhances gas exchange, meeting cellular oxygen demands.
Reduced Contamination Risk
The closed system structure helps prevent external contamination.
Versatile Applications
Suitable for suspension cells, adapted adherent cells, and various upstream process development workflows.
The Erlenmeyer Shake Flask is an indispensable tool in cell culture applications. Its optimal performance relies on correct operation and careful handling.
By following the guidelines above, you can maximize culture efficiency and ensure experimental consistency.
If you are looking for high-quality Erlenmeyer Shake Flasks, feel free to contact us for detailed product information and technical support.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.