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Digestion steps of cells in cell culture flasks

Tue Nov 15 10:41:18 CST 2022

Cell culture flasks are mostly used for culturing adherent cells. When the cells grow to a certain extent, they need to be digested for the next step of passage or other operations. Since they are adherent cells, they need to use digestive enzymes to detach the cells from the bottle wall when digesting the cells. The specific operation steps are as follows:

1. Aspirate the culture medium, and wash the cells once with sterile PBS, Hanks solution or serum-free medium to remove residual serum.

  FuDau T75-Cell-Culture-Flasks

2. Add a small amount of trypsin cell digestion solution to slightly cover the cells. According to the difference in the efficiency of trypsin, the amount of trypsin can be increased or decreased. Leave at room temperature for 30 seconds to 2 minutes (Different cells have different digestion times)

3. Observing under a microscope, the cells shrank significantly, and the bottom of the cell culture flask was observed with the naked eye to find that the shape of the cells changed significantly, showing white; or blowing the cells with a gun found that the cells could just be blown off.

      FuDau T225-Cell-Culture-Flasks

4. At this time, remove the trypsin cell digestion solution. Add complete cell culture medium containing serum, blow off the cells, and then use them directly in subsequent experiments. If digestion is found to be insufficient, add trypsin cell digestion solution to re-digest.

The above are the digestion steps of the cells in the cell culture flask. During the operation, we should pay attention to control the digestion time to avoid the corresponding digestion effect due to insufficient digestion time; or cause damage to the cells due to excessive digestion and affect the later cells. nourish.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.