Cell culture plays an important role in the pharmaceutical industry, vaccine development and other biomanufacturing fields. Cell growth requires nutrients such as culture medium, and the basic requirement for these nutrients is the absence of cytotoxicity and other factors that affect cell growth. How to detect the cytotoxicity of the square media bottles packaged as the media?
Cytotoxicity is the action of chemical substances (drugs) on the basic structure and/or physiological processes of cells, such as cell membrane or cytoskeleton structure, metabolic process of cells, synthesis, degradation or release of cell components or products, ion regulation and cell division, etc. , leading to the disturbance of cell survival, proliferation and/or function, and the adverse reactions caused by it.
Cytotoxicity detection of square media bottles is mainly based on the change of cell membrane permeability, and the following methods are commonly used:
1. MTT and XTT methods: Using the activity of mitochondrial internal enzymes, specific tetrazolium salts can be converted, and then detected by a microplate reader.
2. The method of LDH: the cytotoxicity is detected by detecting the enzyme activity of LDH in the cell culture supernatant.
3. Other enzyme methods: such as detecting the activity of alkaline phosphatase and acid phosphatase in the supernatant.
The above are several detection methods of cytotoxicity. In addition to meeting the requirements of non-cytotoxicity, the square media bottle also needs to meet the requirements of no DNase and no RNase to ensure a good environment for cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.