The state of cell growth is affected by various factors. These factors seem inconspicuous, but if they are not paid attention to, they will cause serious damage to the cells. So, how do these small details affect cell growth, and how can we avoid pitfalls when culturing cells? Today's Fudau class will take you to learn the factors that affect cell growth.
The degree of contact between cells:
Normal cells (diploid) have adherent contact inhibition, and cell growth stops after overgrowth, while abnormal cells such as tumor cells have weak contact inhibition and can grow to a higher cell density, but some cells grow excessively, Its damage is irreversible, so it is not recommended to have too high a cell density when culturing cells.
Key points: Generally, the cells can be passaged when the confluence reaches 70-80%, and they should not grow too full.
Cell growth attachment:
Under normal circumstances, adherent cells will adhere to the wall, but some cells themselves are not firmly adhered to the wall or have poor adhesion. At this time, the coating attachment can promote the adhesion of the cells. Therefore, when cultivating some special cells, it is necessary to adjust the culture flask / dish Use after coating. The commonly used matrix is a mixture of polylysine (D-type, L-type), gelatin, collagen, and extracellular matrix, which can be selected according to needs during culture.
Key points: Matrix-coated culture flasks/dishes can be used when cells adhere poorly.
pH value of medium:
Most cell lines grow best when the pH is around 7.4, and the indicator of conventional medium is phenol red, so in most cases, the medium we see is red, but the color of different mediums will be different, For example DMEM is a bit darker than 1640. When culturing cells, the state of the cells can also be judged according to the color of the culture medium. For example, as the cells grow, the color of the culture medium will become lighter and yellower.
Key points: pay attention to the color change of the medium when culturing cells, and deal with any abnormalities in time.
For most human cells and warm-blooded animal cell lines, the more suitable culture temperature is 37°C, because this temperature is close to body temperature, but it can be slightly lower for safety, and the temperature above body temperature is better than that below body temperature. Temperature is more likely to cause problems. For poultry cells, due to their high body temperature, the culture temperature can be set at about 38.5°C.
Key points: keep the temperature stable when culturing cells, do not open the door of the incubator for a long time, and do not stack a large number of culture vessels in the incubator to avoid air circulation.
Gas phase components:
Most media are suitable for 95% air 5% CO2, but some media are exceptions. For example, L15 medium is suitable for 100% air. CO2 will cause the pH of the culture solution to decrease (the culture solution turns yellow), which will seriously affect the growth of cells; if If you need to use L15 medium to culture cells, but there is no 100% air incubator, you can tighten the bottle cap or change the medium (the effect may be better) for culture. The culture medium suitable for CO2 environment needs to keep the culture bottle ventilated.
Key points: When cultivating cells, pay attention to the selection of medium according to the instructions, and pay attention to the culture environment.
Of course, there are many factors that affect cell growth, such as whether the cell culture consumables are properly selected and whether the quality is qualified. FUDAU BIOTECH focuses on the R&D, production and sales of high-end biological consumables. All products are produced in accordance with c-GMP standards and follow the requirements of ISO9001 and ISO13485 quality management systems. From raw materials, production molding, surface treatment, sealing testing, inner packaging, outer packaging , sterilization and other aspects are controlled at every level to ensure the final quality.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.