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Aseptic operation when culturing cells in cell culture flasks

Mon Nov 28 09:32:20 CST 2022

Cells are very susceptible to the influence of the external environment, resulting in non-adherence, vacuolation, etc. Therefore, we should strictly abide by the principle of aseptic operation when using cell culture flasks to culture cells:

1. Always wipe your hands and work area with 70% ethanol or a suitable disinfectant before experimenting.

2. Before putting cell culture flasks and other experimental equipment into the cell culture fume hood, wipe the outside with 70% ethanol.

3. Do not pour media and reagents directly from the cell culture flask.

cell culture flasks

  T75-TC-Treated-Cell-Culture-Flasks

4. When using sterile glass or disposable plastic pipettes and pipettes to manipulate liquids, each pipette can only be used once to avoid cross-contamination. Do not open sterile pipettes until they are ready for use. Pipettes should be stored in the work area.

5. The culture bottle should be covered after use to prevent the entry of microorganisms and suspended pollutants.

6. The lids of the experimental instruments involved should not be opened until they are used, and should not be exposed to the environment. After use, the cap should be closed immediately.

7. After the cover is removed, it must be placed on the work surface with the opening facing down.

cell culture flasks

   T225-TC-Treated-Cell-Culture-Flasks

8. Sterile utensils and other equipment must be used.

9. Run experiments as quickly as possible to reduce the risk of contamination.

The above are the main points of aseptic operation when culturing cells in cell culture flasks, and they are also issues that need to be paid attention to in cell culture experiments.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.