Application of high-efficiency erlenmeyer shake flask-CHO cell antibody pharmaceutical

Wed Dec 22 11:57:36 CST 2021

High-efficiency erlenmeyer shake flask is a common cell culture consumable, mainly used for the cultivation of mammalian cells, insect cells and other suspension cells. Among them, CHO cell antibody pharmaceutical is an important aspect of its application field.

CHO cells were first isolated by the Puck laboratory in 1957 and obtained by enzymatic digestion of 0.1 g of Chinese hamster ovary tissue. Due to complex post-translational modifications similar to humans, and rapid and robust suspension growth in serum-free and chemically defined media, CHO cells are often used to express active biological macromolecular proteins. At present, nearly 70% of therapeutic proteins that have been marketed are expressed and produced by CHO cells, and CHO cells have become the main tool for the production of therapeutic proteins that require complex post-translational modifications. For example, the recombinant new coronavirus vaccine (CHO cell) launched in March 2021 was developed from CHO cells. The principle is to recombine the new coronavirus S protein receptor binding region (RBD) gene into Chinese hamster ovary (CHO) cell genes, express in vitro to form RBD dimer, and add aluminum hydroxide adjuvant to improve immunogenicity .

Application of high-efficiency erlenmeyer shake flask-CHO cell antibody pharmaceutical

In the early stage of CHO cell antibody pharmaceutical research, high-efficiency erlenmeyer shake flasks are commonly used consumables. After the resuscitated cells are cultured in a high-efficiency shake flask for 2-3 days, the cell density increases and the nutrients are gradually consumed. At this time, the cells need to be expanded. Calculate the expansion volume according to the cell density. When the number of cells to be inoculated in the reactor is reached, stop the shake flask culture, inoculate the reactor for culture, and harvest the cells after the culture is completed.

CHO cell antibody pharmacy is only one aspect of the application of high-efficiency erlenmeyer shake flasks. In addition, this consumable plays an important role in the research of monoclonal antibodies and the fermentation and cultivation of bacteria in dairy factories.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.