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Application of erlenmeyer shake flasks in protein expression

Wed Nov 09 11:05:27 CST 2022

Protein expression refers to a molecular biology technology that expresses foreign gene proteins by model organisms such as bacteria, yeast, animal cells or plant cells, and occupies a core position in genetic engineering technology. Erlenmeyer shake flask is a consumable often used in protein expression.

Protein expression is the process in which genes on DNA are transcribed into RNA and then translated into proteins. Protein expression can express the corresponding (genetic) traits controlled by genes. According to the host cells, protein expression systems can be divided into two categories: prokaryotic expression systems and eukaryotic expression systems. Prokaryotic expression systems include Escherichia coli expression system, Bacillus expression system, Streptomyces expression system. Eukaryotic expression systems include yeast expression systems, insect expression systems, mammalian expression systems and plant cell expression systems.

              FuDau 125ml Erlenmeyer Shake Flasks

The process of prokaryotic protein expression includes the construction of prokaryotic expression vector, transformation into high-efficiency expression host bacteria, induction and expression of target protein, expansion of bacteria, preparation of crude extract of bacterial protein, purification of target protein, etc. It is used in the culture and expansion stage of bacteria. If it is a large-scale protein expression, a larger-capacity high-efficiency shake flask needs to be used.

        FuDau 1000ml Erlenmeyer Shake Flasks 

Protein expression is only one aspect of the application of erlenmeyer shake flasks. In addition, it is also used for the culture of suspension cells, the preparation or storage of media, and is an economical and affordable culture or storage container.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.