Application of cell factory in the preparation of forest encephalitis inactivated vaccine

Thu Jun 23 11:01:56 CST 2022

As a multi-layer cell culture vessel, cell factories have a very wide range of applications. This cell culture consumable is also used in the large-scale production of vaccines, such as forest encephalitis inactivated vaccines.

Forest encephalitis is an acute infectious disease of the central nervous system caused by the forest encephalitis virus, which is transmitted to humans through the bite of blood-sucking insects (ticks). Fever, headache, nausea, vomiting, confusion, and neck stiffness are present at the onset. Later, muscle paralysis of the neck, shoulders and upper limbs reappeared, manifested as weakness in lifting the head, drooping shoulders, and swaying with weakness in both hands, etc. The recovery period was long, and the sequelae of paralysis could be left. Forest encephalitis inactivated vaccine is inoculated into primary hamster kidney cells with forest encephalitis virus "Sen Zhang" strain, cultured, harvested virus liquid, inactivated and purified, and then added stabilizer and aluminum hydroxide adjuvant. to make. It is a milky white suspension, containing thimerosal as a preservative.

cell factories

The cell factory is mainly used in the culture of primary hamster kidney cells. As an adherent cell, it has higher requirements on the surface of the culture vessel. In order to facilitate the better adherence of cells to the wall, the surface of the culture vessel needs to be modified to introduce hydrophilic groups to enhance the ability of cell adsorption and protein binding, making it suitable for the growth and reproduction of primary hamster kidney cells.

In terms of specifications, 10-layer and 40-layer cell factories are used in the preparation of forest encephalitis inactivated vaccines, which have a larger culture area and are suitable for large-scale production and preparation of vaccines.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.