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Application of cell factory in live attenuated measles vaccine

Tue Jun 28 10:27:46 CST 2022

With the continuous advancement of biotechnology, large-scale culture containers such as cell factories have also been applied to more and more fields, such as biopharmaceuticals, vaccine preparation, etc. This cell culture consumable is used in the preparation of live attenuated measles vaccine.

Measles is an acute systemic exanthematous respiratory infectious disease caused by measles virus. Measles is prevalent in the age group of 1-5, accounting for about 80% of the total incidence. Typical measles are characterized by fever, conjunctivitis, inflammation of the upper respiratory tract, Kochi's spots on the buccal mucosa, maculopapular rash on the whole body, and pigmented spots in the rash. Improving the specific immunity level of the population is the leading measure to prevent measles. The measles vaccine is prepared by inoculating chicken embryo cells with attenuated measles virus strains, culturing and harvesting the virus liquid and then freeze-drying. Add sterile water for injection according to the amount indicated on the ampoule label and use it after it is completely dissolved. Injection into the outer deltoid muscle attachment of the upper arm can prevent measles.

The production process of live attenuated measles vaccine includes cell culture, virus inoculation, virus culture, virus harvest and purification, adjuvant configuration and mixing, etc. The cell factory is mainly used in the cell culture stage. In the mass production stage, a 10- or even 40-story factory is required, and at this time, it is necessary to use automated equipment to operate.

The above is the application of cell factory in the preparation of live attenuated measles vaccine. This cell culture method takes up little space, and can also use a closed pipeline system to reduce pollution and become a commonly used container for large-scale cell culture.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.