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Application of PETG Media Bottle for Kanamycin Sulfate Solution

Mon Jul 18 11:22:32 CST 2022

In the process of cell culture, various solutions and reagents are needed. They either provide nutrients for cell growth or inhibit various bacterial contamination, and play an important role in cell culture experiments. PETG media bottles are dedicated packaging for such solutions or reagents, such as kanamycin sulfate solution.

Kanamycin is a broad-spectrum aminoglycoside antibiotic that can be isolated from the bacterium Streptomyces kanamyceticus. By combining with 30S ribosomes, the mRNA code is misread, thereby inhibiting the synthesis of proteins, and it is effective for both Gram-positive and negative bacteria. Kanamycin can be used to inhibit bacterial contamination in cell culture; in molecular biology research, it is often used to selectively screen bacterial clones successfully transformed with kanamycin resistance genes; it is also commonly used in Agrobacterium-mediated transformation experiments, selecting Sexual screening of plant tissues carrying the npt II (APH3) gene.

This solution needs to be stored at -20°C away from light, and should not be stored at room temperature or 4°C for a long time. The raw material of PETG media bottle has good temperature resistance and still has good performance at -70 °C, which is very suitable for storing kanamycin solution and other liquids that need to be stored at low temperature. The bottle body has high transparency, which is convenient to observe the state and capacity of the solution.

It should be noted that the kanamycin sulfate solution needs to be thawed at 2°C to 8°C and used after shaking. Do not freeze and thaw repeatedly. When the dosage is small, it is recommended to freeze and store in separate packages. Pay attention to aseptic operation when opening the PETG media bottle. 

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.