Analysis of the causes of cell clumping in cell culture flasks

Mon Nov 08 11:50:20 CST 2021

Cultivating cells is a very rigorous task. In addition to creating a good growth environment for cells, it must also promptly resolve various emergencies during the culture process. Cell clumping in cell culture flasks is a phenomenon often encountered. What is the reason for this situation?

The clumping phenomenon of cells in the cell culture flasks can generally be analyzed from the following aspects:

1. The digestion process may be too rough, pipetting too hard, digestion for too long, too strong or toxic digestive juice, etc., will cause cell death. After the cell dies, it releases DNA, entangles other cells, and forms a sticky cell cluster.

2. Excessive cell centrifugation speed or too long time can also easily cause cell clumping.

3. When the digestion is not complete, the connection between the cells and the cell is not separated; when the digestion is over, the round cells tend to pile up and it is difficult to separate again.

4. Cells that are in poor condition and aging may also grow in clumps (such as poor cell condition caused by delay in changing the medium, too long to pass through, pollution, etc.).

Analysis of the causes of cell clumping in cell culture flasks

5. There is no pipetting evenly when passaging, and there are many cell clumps, which will be a clump when cultured.

6. Uneven inoculation or undigested cells.

7. Non-vibration culture or too much bacteria (cells) will form clumps.

If the cells in the cell culture flasks appear clumping, you can analyze the above aspects to find out the reason, and formulate strict procedures before the operation to avoid this phenomenon.

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.