Serum provides nutrients needed for cell growth and is an important solution for culturing cells. Sometimes we will find the phenomenon of precipitation in the PETG media bottle. What is the reason for this?
There are often visible precipitates in serum. There are various types of components, and there are many reasons for them, mainly including fibrin, calcium phosphate and some other components. The formation mechanism of serum precipitation is not very clear. The serum that has just left the factory should be clear and transparent. In order to ensure the quality, the serum will be placed in a PETG media bottle and stored in an environment of -20°C. The main reasons for precipitation are as follows:
1. If the temperature fluctuates during transportation, it is easy to precipitate a large amount of precipitation.
2. Incorrect thawing method can also cause a large amount of precipitation of serum. Serum should be thawed correctly in a step-by-step thawing method: after taking it out from -20°C, it should be thawed slowly in a 4°C refrigerator, and finally thawed at room temperature.
3. Heat inactivation, long-term storage at 2-8°C, gamma irradiation, and long-term storage in a refrigerator that can be automated cream are also likely to cause precipitation in serum.
In conclusion, precipitation in PETG media bottles is difficult to predict and avoid, and there is no need to worry about precipitation, which does not affect the quality of the serum. Find out the cause of the precipitation and pay more attention in the operation and storage, you can well avoid the precipitation.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.