Rekwiżiti ta 'ċelluli aderenti għal konsumabbli tal-kultura taċ-ċelluli

Wed May 18 12:12:10 CST 2022

Ħafna miċ-ċelloli kkultivati ​​in vitro huma ċelloli aderenti. It-tkabbir ta 'ċelluli bħal dawn għandu jkollu wiċċ ta' appoġġ li jista 'jitwaħħal miegħu. Iċ-ċelloli jistgħu jikbru u jimmultiplikaw fuq il-wiċċ billi jiddependu fuq fatturi ta 'twaħħil mnixxija minnhom infushom jew ipprovduti fil-medju tal-kultura. Allura, x'inhuma r-rekwiżiti għall-konsumabbli tal-kultura taċ-ċelluli ta 'ċelloli aderenti?

Iċ-ċelloli dipendenti mill-ankraġġ għandhom jitwaħħlu mal-ħajt tar-reċipjent tal-kultura (flixkun) meta jiġu kkultivati. Ladarba ċ-ċelloli jitwaħħlu, jinfirxu malajr, imbagħad jibdew il-mitożi, u jidħlu malajr fil-fażi tat-tkabbir logaritmiku. Wara ftit jiem, iċ-ċelloli se jkopru l-wiċċ tar-reċipjent tal-kultura u jiffurmaw monosaff taċ-ċelluli dens. Il-konsumabbli tal-kultura taċ-ċelluli użati għal ċelluli aderenti jinkludu fabbriki taċ-ċelluli, pjanċi tal-kultura taċ-ċelluli, fliexken tal-ispinner taċ-ċelluli, eċċ. Fil-preżent, il-biċċa l-kbira tal-prodotti tal-konsum taċ-ċelluli fis-suq huma prinċipalment materjali polimeri bħal PS, PC u PE. Dan it-tip ta 'materjal m'għandux il-proprjetajiet idrofiliċi tal-ħġieġ, u l-wiċċ jeħtieġ li jiġi ttrattat b'mod speċjali qabel ma jkun jista' jintuża għall-kultivazzjoni ta 'ċelloli aderenti. Il-konsumabbli tal-kultura taċ-ċelluli trattati b'mod speċjali għandhom grupp idrofiliku introdott fuq il-wiċċ, li jippermetti li ċ-ċelloli jaderixxu aħjar mal-wiċċ tal-konsumabbli, li jwassal għat-tkabbir taċ-ċelluli u r-riproduzzjoni.

Tkabbir taċ-ċelluli jeħtieġ ambjent speċifiku, bħall-karbonju dijossidu, pH, sterilità, pressjoni osmotika, eċċ., Għalhekk il-konsumabbli tal-kultura taċ-ċelluli għandhom jiġu sterilizzati u jilħqu kundizzjonijiet ħielsa minn DNase u RNase qabel ma jkunu jistgħu jintużaw, sabiex tiġi evitata l-kontaminazzjoni taċ-ċelluli li taffettwa l-progress tal-kultura taċ-ċelluli.@ It-trattament u l-isterilizzazzjoni __@Surface TC huma ż-żewġ rekwiżiti bażiċi għall-konsumabbli tal-kultura taċ-ċelluli għal ċelloli aderenti, u huma wkoll prerekwiżiti għall-progress bla xkiel tax-xogħol tal-kultura taċ-ċelluli. Għalhekk, trid tkun aktar kawt meta tagħżel prodotti konsumabbli.

Surface TC treatment and sterilization are the two basic requirements for cell culture consumables for adherent cells, and they are also prerequisites for the smooth progress of cell culture work. Therefore, you need to be more cautious when choosing consumable products.

 

The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.

The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.

In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.

Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.

NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.

In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.