English
"Effett tat-tarf" hija sitwazzjoni li ta' spiss tiltaqa' magħhom waqt l-użu ta' Plati ta’ 96-il bir, speċjalment f'immunoreazzjonijiet marbuta ma' enzimi (esperimenti ELISA). Din is-sitwazzjoni tikkawża li r-rata tat-tkabbir taċ-ċelloli fit-toqob tat-tarf tonqos, li taffettwa direttament ir-riżultati tat-test.
Din is-sitwazzjoni tista’ tiġi ttrattata bil-modi li ġejjin:
1. Pre-inkubazzjoni: Uża banju ta 'l-ilma jew meta żżid is-soluzzjoni ta' reazzjoni ma 'pjanċa ta' 96 bir, saħħan il-pjanċa u s-soluzzjoni għat-temperatura ta 'inkubazzjoni (bħal 37 ° C), li tista' faċilment telimina l- "effett tat-tarf" u ttejjeb Il- ripetibbiltà tal-kejl. Barra minn hekk, xi studji sabu li t-tqegħid taċ-ċelloli pavimentati f'temperatura tal-kamra għal siegħa u r-ritorn tagħhom fl-inkubatur ta '37 ° C biex ikomplu jikkultivaw wara li ċ-ċelloli jkunu ssetiljaw u aderixxu mal-ħajt jista' wkoll itejjeb b'mod sinifikanti l-effett tat-tarf.
2. Uża kopertura anti-volatili: Xi nies żviluppaw kopertura mtaqqba anti-volatili għall-effett tat-tarf ikkawżat mill-effett ta 'volatilizzazzjoni tal-pjanċa poruża. Il-kopertura tal-pjanċa mtaqqba tista 'tifforma saff tal-fwar tal-ilma saturat fuq il-pjanċa mtaqqba biex issir tarka tal-fwar, u tiżola l-ambjent ġewwa l-pjanċa mtaqqba mill-ambjent tal-laboratorju, sabiex iżżomm aħjar il-pjanċa mtaqqba fi stat relattivament stabbli.
3. Abbanduna t-toqob periferali: Meta l-metodi ta 'hawn fuq ma jistgħux jiġu solvuti, sabiex jinkisbu riżultati tat-test aktar affidabbli, l-aktar metodu sempliċi u sempliċi huwa li ma tużax dawn it-toqob periferali.
Dan ta' hawn fuq huma soluzzjonijiet biex jipprevjenu l-effetti tat-tarf f' Plati ta' 96-il bir. Il-kontroll ta' dawn il-metodi ta' pproċessar jista' jnaqqas l-impatt ta' l-effetti tat-tarf fuq ir-riżultati tat-test.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.