English
Il-kultivazzjoni taċ-ċelloli hija biċċa xogħol rigoruża ħafna. Minbarra l-ħolqien ta 'ambjent ta' tkabbir tajjeb għaċ-ċelloli, għandu wkoll isolvi fil-pront diversi emerġenzi matul il-proċess tal-kultura. Il-ġbir taċ-ċelluli fi Flasks tal-kultura taċ-ċelluli huwa fenomenu li spiss jiltaqgħu magħhom. X'inhi r-raġuni għal din is-sitwazzjoni?
Il-fenomenu ta 'cluping taċ-ċelloli fil-garafini tal-kultura taċ-ċelluli jista' ġeneralment jiġi analizzat mill-aspetti li ġejjin:
1. Il-proċess tad-diġestjoni jista 'jkun mhux maħdum wisq, pipetta iebes wisq, diġestjoni għal żmien twil wisq, meraq diġestiv qawwi wisq jew tossiku, eċċ., Se jikkawża mewt taċ-ċelluli. Wara li ċ-ċellula tmut, tirrilaxxa d-DNA, tħabbel ċelluli oħra, u tifforma grupp taċ-ċelluli li jwaħħlu.
2. Veloċità eċċessiva taċ-ċentrifugazzjoni taċ-ċelluli jew ħin twil wisq jistgħu wkoll faċilment jikkawżaw ċelluli clumping.
3. Meta d-diġestjoni ma tkunx kompluta, il-konnessjoni bejn iċ-ċelloli u ċ-ċellula mhix separata; meta d-diġestjoni tkun spiċċat, iċ-ċelloli tondi għandhom it-tendenza li jinġabru u huwa diffiċli li terġa’ tissepara.
4. Ċelloli li huma f'kundizzjoni ħażina u li qed jixjieħu jistgħu jikbru wkoll f'ċapep (bħal kundizzjoni ħażina taċ-ċelluli kkawżata minn dewmien fil-bdil tal-medju, twil wisq biex jgħaddi minnu, tniġġis, eċċ.).
5. M'hemm l-ebda pipetta b'mod uniformi meta jgħaddi, u hemm ħafna ċapep ta 'ċelluli, li se jkunu ċapep meta kkultivati.
6. Inokulazzjoni irregolari jew ċelluli mhux diġeriti.
7. Kultura mhux vibrazzjoni jew batterji wisq (ċelluli) se jiffurmaw ċapep.
Jekk iċ-ċelloli fil-kunjetti tal-kultura taċ-ċelluli jidhru magħquda, tista 'tanalizza l-aspetti ta' hawn fuq biex issir taf ir-raġuni, u tifformula proċeduri stretti qabel l-operazzjoni biex tevita dan il-fenomenu.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.