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2. Bahan dan Kaedah
2.1. Penyelenggaraan kultur sel Huh7.5
Huh7.5 dikekalkan dalam kelalang T kultur sel dalam medium yang mengandungi serum (SCM): Medium Helang Modified Dulbecco (DMEM) (Gibco, Paisley, UK) yang mengandungi 4 mM GlutaMAX dan ditambah dengan 10% serum lembu janin (Sigma, St. Louis, MO, Amerika Syarikat), 100 U/mL penicillin dan 100 µg/mL streptomycin (Sigma). Dalam kelalang T175 (Nunc, Roskilde, Denmark) dan kelalang kultur tiga lapisan (Nunc), sel Huh7.5 telah dilalui setiap 2-3 hari. Semua kultur sel dikekalkan pada 37 °C dan 5% CO2.
2.2. Stok Virus HCV
Dua stok yang disahkan turutan genotip 5a rekombinan SA13/JFH1core-NS5B [28], ditanam dalam kultur sel monolayer Huh7.5 dalam kelalang T, telah digunakan dalam kajian ini. Stok virus laluan ketiga dengan titer infektiviti 5.8 log10 FFU/mL, ditanam di bawah keadaan yang mengandungi serum (SC) [39], digunakan untuk menyuntik kultur CelCradle™, kultur kelalang goncang dan untuk eksperimen pencirian virus. Stok virus laluan keempat dengan titer infektiviti 5.1 log10 FFU/mL [32], ditanam dalam keadaan SF [39], telah digunakan untuk eksperimen pencirian virus.
Sumber daripada: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.